PMID- 8947879
OWN - NLM
STAT- MEDLINE
DCOM- 19970227
LR  - 20191101
IS  - 1352-0504 (Print)
IS  - 1352-0504 (Linking)
VI  - 3
IP  - 6
DP  - 1996 Nov
TI  - Hepatitis C virus infection and mixed cryoglobulinaemia: assessment of HCV RNA 
      copy numbers in supernatant, cryoprecipitate and non-liver cells.
PG  - 285-92
AB  - The aim of this study was to characterize hepatitis C virus (HCV) infection in 
      patients with mixed cryoglobulinaemia (MC). The HCV RNA copy number was assayed 
      in clinical specimens from 15 consecutive patients with MC and HCV infection. 
      Absolute quantification of HCV RNA molecules was performed using a competitive 
      reverse transcription-polymerase chain reaction (cRT-PCR). Specific HCV RNA 
      sequences were detected and quantified in plasma samples from all patients (mean 
      HCV RNA copy number 4.9 x 10(6) ml-1 plasma). A high concentration of HCV RNA 
      molecules was detected in the cryoprecipitates of eight of the 15 patients, who 
      had a cryoprecipitate/supernatant ratio higher than 3.0 (range 3.60 to 186.80): 
      in the remaining seven patients this ratio was close to or lower than 1.0 (range 
      0.13 to 1.60). Quantitative analysis of HCV RNA molecules in cells other than 
      hepatocytes (i.e. peripheral blood mononuclear cells (PBMCs) and bone marrow 
      cells (BMCs), in which the HCV replicative intermediate was detected using strand 
      specific RT-PCR, demonstrated that infection is detectable in nearly 60% of these 
      extrahepatic cells. Quantitative analysis of HCV RNA in PBMCs and BMCs revealed 
      low levels of viral nucleic acids.
FAU - Manzin, A
AU  - Manzin A
AD  - Istituti di Microbiologia e di Clinica Medica, Universita di Ancona, Italy.
FAU - Solforosi, L
AU  - Solforosi L
FAU - Candela, M
AU  - Candela M
FAU - Cherubini, G
AU  - Cherubini G
FAU - Piccinini, G
AU  - Piccinini G
FAU - Brugia, M
AU  - Brugia M
FAU - Gabrielli, A
AU  - Gabrielli A
FAU - Clementi, M
AU  - Clementi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Viral Hepat
JT  - Journal of viral hepatitis
JID - 9435672
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Aged
MH  - Bone Marrow Cells
MH  - Cryoglobulinemia/*complications
MH  - Female
MH  - Gene Dosage
MH  - Genotype
MH  - Hepacivirus/*genetics/isolation & purification
MH  - Hepatitis C/blood/complications/pathology/*virology
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Middle Aged
MH  - RNA, Viral/*analysis
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1111/j.1365-2893.1996.tb00100.x [doi]
PST - ppublish
SO  - J Viral Hepat. 1996 Nov;3(6):285-92. doi: 10.1111/j.1365-2893.1996.tb00100.x.