PMID- 8948087
OWN - NLM
STAT- MEDLINE
DCOM- 19970228
LR  - 20061115
IS  - 0049-8254 (Print)
IS  - 0049-8254 (Linking)
VI  - 26
IP  - 11
DP  - 1996 Nov
TI  - Design, production and characterization of antibodies discriminating between the 
      phenol- and monoamine-sulphating forms of human phenol sulphotransferase.
PG  - 1113-9
AB  - 1. Phenol sulphotransferases (PSTs) are important enzymes in xenobiotic and 
      endobiotic detoxication, and a key component of the body's chemical defence 
      mechanism. 2. Human phenol-(P-PST) and monoamine-(M-PST) sulphating forms of PST 
      share 93% amino acid sequence identity, and to date the various antibodies 
      produced against PSTs all recognize both enzymes. 3. We have identified two 
      peptides based on the cDNA-derived amino acid sequences of human P-PST and M-PST, 
      which elicited for the first time antibodies capable of discriminating between 
      these highly homologous enzymes. 4. These antibodies represent valuable tools for 
      studying the expression, distribution and function of human phenol 
      sulphotransferases.
FAU - Rubin, G L
AU  - Rubin GL
AD  - Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and 
      Medical School, Dundee, UK.
FAU - Sharp, S
AU  - Sharp S
FAU - Jones, A L
AU  - Jones AL
FAU - Glatt, H
AU  - Glatt H
FAU - Mills, J A
AU  - Mills JA
FAU - Coughtrie, M W
AU  - Coughtrie MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Xenobiotica
JT  - Xenobiotica; the fate of foreign compounds in biological systems
JID - 1306665
RN  - 0 (Antibodies)
RN  - 0 (Peptides)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies/*analysis
MH  - Arylsulfotransferase/immunology/*metabolism
MH  - Humans
MH  - Immunoblotting
MH  - Molecular Sequence Data
MH  - Peptides/*immunology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.3109/00498259609050256 [doi]
PST - ppublish
SO  - Xenobiotica. 1996 Nov;26(11):1113-9. doi: 10.3109/00498259609050256.