PMID- 8948504
OWN - NLM
STAT- MEDLINE
DCOM- 19970313
LR  - 20190826
IS  - 0090-6980 (Print)
IS  - 0090-6980 (Linking)
VI  - 52
IP  - 5
DP  - 1996 Nov
TI  - Mechanism of action of TNF-alpha-stimulated prostaglandin production in cultured 
      bovine luteal cells.
PG  - 361-73
AB  - Tumor necrosis factor-alpha (TNF-alpha) influences hormone synthesis of many 
      ovarian cell types and can also exert cytotoxic effects, possibly by increasing 
      the synthesis of prostaglandins. The purpose of the present study was to 
      characterize the mechanism of TNF-alpha-stimulated prostaglandin; F2 alpha (PGF2 
      alpha) production in cultured bovine luteal cells. Inhibitors of RNA and protein 
      synthesis (actinomycin D and cycloheximide, respectively) completely blocked 
      TNF-alpha-stimulated PGF2 alpha production. The phospholipase A2 inhibitor, 
      aristolochic acid, prevented TNF-alpha-stimulated, but not basal, PGF2 alpha 
      production, whereas the phospholipase C inhibitor, compound 48/80, was without 
      effect. The addition of arachidonic acid to cultures did not overcome the 
      inhibitory effects of cycloheximide or aristolochic acid. In conclusion, 
      TNF-alpha-stimulated prostaglandin production by bovine luteal cells is dependent 
      upon the stimulation of phospholipase A2 through mechanisms which require 
      synthesis of RNA and protein.
FAU - Townson, D H
AU  - Townson DH
AD  - Department of Animal Sciences, Ohio State University, Columbus 43210 USA.
FAU - Pate, J L
AU  - Pate JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Prostaglandins
JT  - Prostaglandins
JID - 0320271
RN  - 0 (Aristolochic Acids)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phenanthrenes)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 94218WFP5T (aristolochic acid I)
RN  - 98600C0908 (Cycloheximide)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - EC 3.1.4.- (Type C Phospholipases)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/pharmacology
MH  - *Aristolochic Acids
MH  - Cattle
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Corpus Luteum/cytology/*drug effects/*metabolism
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Dinoprost/*biosynthesis
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Phenanthrenes/pharmacology
MH  - Phospholipases A/antagonists & inhibitors
MH  - Phospholipases A2
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Type C Phospholipases/antagonists & inhibitors
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S0090698096001049 [pii]
AID - 10.1016/s0090-6980(96)00104-9 [doi]
PST - ppublish
SO  - Prostaglandins. 1996 Nov;52(5):361-73. doi: 10.1016/s0090-6980(96)00104-9.