PMID- 8950606
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20191101
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 106
IP  - 5
DP  - 1996 Nov
TI  - Combined immunophenotyping and FISH with sex chromosome-specific DNA probes for 
      the detection of chimerism in epidermal Langerhans cells after sex-mismatched 
      bone marrow transplantation.
PG  - 481-5
AB  - Langerhans cells (LC) of the skin represent bone marrow-derived dendritic 
      antigen-presenting cells and are therefore important in pathophysiological 
      processes such as rejection, graft-versus-host disease, and 
      graft-versus-leukemia-reaction after bone marrow transplantation (BMT). For 
      understanding of these diseases, the evaluation of the chimeric status of LC 
      following BMT is of great interest. To analyze the sex chromosome constitution of 
      LC in the skin, we established a modified and refined technique of combined 
      immunophenotyping and fluorescence in situ hybridization (FISH) and investigated 
      frozen sections of skin biopsies from nine patients after allogeneic 
      sex-mismatched BMT and of two healthy donors for control. LC were specifically 
      labeled using a fluorescent CD1 a antibody and hybridized simultaneously with X 
      and Y chromosome-specific DNA probes. The results of this practical application 
      on nine leukemia patients show the appearance of donor-type LC and the 
      persistence of host-type LC at various times (36 up to 1395 days) after 
      sex-mismatched BMT. Complete chimerism of LC could not be detected in any case. 
      The frequency of recipient-specific LC ranged from 7% to 92% and showed no 
      correlation with time postgrafting. We conclude from our results of 1461 analyzed 
      LC that combined immunophenotyping and interphase cytogenetic analysis by FISH is 
      the method of choice for the assessment of chimerism in a particular cell type 
      after sex-mismatched BMT. Its practical application on other tissues affected by 
      BMT-related pathophysiological processes reveals further knowledge of the 
      time-dependent course of chimeric patterns after BMT.
FAU - Hessel, H
AU  - Hessel H
AD  - GSF-National Research Center for Environment and Health, Institute of 
      Radiobiology.
FAU - Mittermuller, J
AU  - Mittermuller J
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Weier, H U
AU  - Weier HU
FAU - Bauchinger, M
AU  - Bauchinger M
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (DNA Probes)
SB  - IM
MH  - Acute Disease
MH  - *Bone Marrow Transplantation
MH  - *Chimera
MH  - DNA Probes/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Langerhans Cells/*chemistry
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy
MH  - Leukemia, Myeloid/therapy
MH  - Male
MH  - Myelodysplastic Syndromes/therapy
MH  - Phenotype
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy
MH  - *Sex Chromosomes
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF02473310 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 1996 Nov;106(5):481-5. doi: 10.1007/BF02473310.