PMID- 8955048
OWN - NLM
STAT- MEDLINE
DCOM- 19970108
LR  - 20231213
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 226
IP  - 2
DP  - 1996 Dec 15
TI  - Molecular studies on bromovirus capsid protein. I. Characterization of 
      cell-to-cell movement-defective RNA3 variants of brome mosaic virus.
PG  - 281-93
AB  - To evaluate the extent to which brome mosaic virus (BMV) coat protein (CP) gene 
      is involved in the process of cell-to-cell movement. Chenopodium quinoa plants 
      were coinoculated with BMV wild-type RNAs 1 and 2 and a RNA3 variant containing 
      either the beta-glucuronidase (GUS) gene in place of CP (B3/CP-GUS), to be 
      subjected to GUS analysis, or a deletion in the CP gene to be analyzed by 
      fluorescence in situ hybridization (FISH). Irrespective of time postinoculation, 
      GUS expressed from the subgenomic mRNA of B3/CP-GUS was restricted to initially 
      infected, single epidermal cells. The defective cell-to-cell movement exhibited 
      by B3/CP-GUS was not complemented in trans when transcripts of B3 delta MP, a 
      RNA3 variant capable of synthesizing functional wt CP but not movement protein 
      (MP), were added to the inoculum. Application of FISH, a technique versatile in 
      discriminating subliminal infections from efficient cell-to-cell spread in 
      nonpermissive and permissive hosts, respectively, to leaves inoculated with BMV 
      RNA3 variants defective in CP synthesis, confirmed that the resulting infections 
      were subliminal. These data provide direct evidence for the requirement of an 
      encapsidation-competent CP to be expressed in conjunction with a functional MP 
      for efficient cell-to-cell spread of BMV.
FAU - Schmitz, I
AU  - Schmitz I
AD  - Department of Plant Pathology, University of California, Riverside 92521-0122, 
      USA.
FAU - Rao, A L
AU  - Rao AL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Bromovirus/genetics/*physiology
MH  - Capsid/genetics/*physiology
MH  - Edible Grain
MH  - Genetic Complementation Test
MH  - Glucuronidase
MH  - Hordeum
MH  - In Situ Hybridization, Fluorescence
MH  - Mutagenesis
MH  - Plants, Toxic
MH  - Nicotiana
EDAT- 1996/12/15 00:00
MHDA- 1996/12/15 00:01
CRDT- 1996/12/15 00:00
PHST- 1996/12/15 00:00 [pubmed]
PHST- 1996/12/15 00:01 [medline]
PHST- 1996/12/15 00:00 [entrez]
AID - S0042-6822(96)90656-X [pii]
AID - 10.1006/viro.1996.0656 [doi]
PST - ppublish
SO  - Virology. 1996 Dec 15;226(2):281-93. doi: 10.1006/viro.1996.0656.