PMID- 8955577
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20190830
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 26
IP  - 11
DP  - 1996 Nov
TI  - Codfish allergy in adults. Specific tests for IgE and histamine release vs 
      double-blind, placebo-controlled challenges.
PG  - 1276-85
AB  - BACKGROUND: At present, several in vitro tests for immunoglobulin E 
      (IgE)-mediated food allergy are available. An estimation of the diagnostic 
      accuracy of the various tests used in predicting clinical sensitivity to codfish 
      in a well-characterized allergic material is necessary. OBJECTIVES: To compare 
      the diagnostic value of four specific IgE tests, and histamine release from 
      basophils (HR) in identifying clinical type I allergy to codfish. As a true 
      diagnosis, double-blind, placebo-controlled food challenges (DBPCFC) were 
      employed. METHODS: Eight clinically codfish-allergic adult patients were 
      investigated together with 30 codfish-tolerant control subjects for evidence of 
      codfish-specific reactivity by Phadebas RAST (PHA), Pharmacia CAP System RAST 
      (CAP), Magic Lite (ML) and HR. To characterize the diagnostic properties of a 
      freshly prepared raw codfish extract, experiments were conducted employing an 
      in-house radioallergosorbent test (RAST), the Maxisorp RAST (MAXI) and HR. 
      Finally, protein profile and IgE-reacting allergens were detected by means of 
      sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 
      immunoblotting. RESULTS: The sensitivities of HR with commercial extract and the 
      three commercially available specific IgE analyses were 0.83 and 1.00 
      respectively. Specificities were 1.00 (HR) and 0.87-1.00 (specific IgE tests). 
      Freshly prepared codfish extracts improved the sensitivity of HR. SDS-PAGE 
      revealed approximately 29 bands (< 14.3-200 kDa) including a band of 12-13 kDa, 
      and in immunoblotting 18 sera identified 17 IgE-binding bands. The protein 
      migrating at 12-13 kDa was identified in the fresh codfish extract tested with 
      sera from all clinical codfish allergics, while no significant reaction was seen 
      in the control subjects. CONCLUSION: Based on the small number of adult patients 
      included in our study, the in vitro assays with commercial and fresh extracts 
      have high sensitivity and are acceptable for screening for codfish allergy. 
      Specificity of Phadebas, CAP, and our in-house RAST was less than unity, whereas 
      ML and strong binding of IgE to a 12-13 kDa protein completely matches DBPCFC 
      results, and thus seems sufficient for establishing the diagnosis.
FAU - Hansen, T K
AU  - Hansen TK
AD  - Food Allergy Unit, National University Hospital, Copenhagen, Denmark.
FAU - Bindslev-Jensen, C
AU  - Bindslev-Jensen C
FAU - Skov, P S
AU  - Skov PS
FAU - Poulsen, L K
AU  - Poulsen LK
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Epitopes)
RN  - 0 (Tissue Extracts)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Double-Blind Method
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes
MH  - Fishes/*immunology
MH  - Food Hypersensitivity/diagnosis/*etiology
MH  - Histamine Release
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/blood/immunology
MH  - Skin Tests
MH  - Sodium Dodecyl Sulfate
MH  - Tissue Extracts/immunology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1111/j.1365-2222.1996.tb00525.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 1996 Nov;26(11):1276-85. doi: 
      10.1111/j.1365-2222.1996.tb00525.x.