PMID- 8956370
OWN - NLM
STAT- MEDLINE
DCOM- 19970312
LR  - 20061115
IS  - 1081-650X (Print)
IS  - 1081-650X (Linking)
VI  - 108
IP  - 6
DP  - 1996 Nov
TI  - Antibodies to beta 2 glycoprotein I: standardized immunoassay and a reference 
      interval for healthy controls.
PG  - 467-72
AB  - Patients with antiphospholipid syndrome (APS) demonstrate an antibody reactivity 
      with beta 2 glycoprotein I (beta 2 GPI) independent of the anionic phospholipids 
      that previously had been believed to be the relevant autoantigens associated with 
      this syndrome. Immunoassays for IgG anti-beta 2 GPI have, however, suffered from 
      a lack of standardization. In this article, we describe an assay based on 
      reference standards that we developed recently. The assay exhibits excellent 
      linearity, with regard to both application of the standards and dilution of 
      out-of-range specimens. Precision was assessed both within the between run and 
      was judged to be satisfactory. A reference interval was developed on the basis of 
      a control group consisting of 111 men and 93 women, yielding a range of 0-19 
      standard IgG anti-beta 2 GPI units (SGU) for the ninety-fifth percentile of 
      values. These data suggest that this standardized anti-beta 2 GPI assay may be 
      useful in the clinical diagnostic laboratory.
FAU - Najmey, S S
AU  - Najmey SS
AD  - Department of Medicine, Seton Hall University School of Graduate Medical 
      Education, St. Joseph's Hospital and Medical Center, Paterson, NJ 07503, USA.
FAU - Erickson, E N Jr
AU  - Erickson EN Jr
FAU - Keil, L B
AU  - Keil LB
FAU - DeBari, V A
AU  - DeBari VA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Assoc Am Physicians
JT  - Proceedings of the Association of American Physicians
JID - 9514310
RN  - 0 (Autoantibodies)
RN  - 0 (Glycoproteins)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Antiphospholipid Syndrome/blood/*immunology
MH  - Autoantibodies/*analysis/blood/immunology
MH  - Female
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Immunoassay/*standards
MH  - Male
MH  - Sensitivity and Specificity
MH  - beta 2-Glycoprotein I
EDAT- 1996/11/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - Proc Assoc Am Physicians. 1996 Nov;108(6):467-72.