PMID- 8956864
OWN - NLM
STAT- MEDLINE
DCOM- 19961230
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 92
IP  - 1
DP  - 1996 Nov
TI  - The detection of trisomies 8 and 9 in patients with essential thrombocytosis by 
      fluorescence in situ hybridization.
PG  - 14-7
AB  - Essential thrombocytosis (ET) is a clonal, chronic myeloproliferative disorder 
      (MPD) originating from a multipotent stem cell. To date no specific cytogenetic 
      marker has been found in ET. It was recently reported that chromosomal 
      aberrations have been detected by fluorescence in situ hybridization (FISH) in 
      patients with normal karyotypes or nonanalyzable metaphases. Therefore, we 
      evaluated whether trisomies 8 and 9, which are commonly found in MPDs, can be 
      detected in ET by FISH and compared the results with chromosome analysis. 
      Peripheral blood mononuclear cells of patients with essential thrombocytosis were 
      studied by classical chromosome banding and by FISH. We used biotin labeled alpha 
      satellite of chromosome 8 and biotin labeled beta satellite of chromosome 9 as 
      probes for the FISH studies. FISH detected 5 patients with trisomy 8 and 5 with 
      trisomy 9 of the 18 patients evaluated. No trisomy was found by cytogenetic 
      studies. The trisomies were detected by FISH in only a minority of the cells. No 
      correlation was found between the presence of a trisomy and clinical 
      characteristics. FISH is a sensitive method for the detection of trisomies 8 and 
      9 in patients with ET. The common finding of these chromosomal aberrations in MPD 
      suggests that genes associated with myeloid proliferation are located on these 
      chromosomes. Standardization of interphase cytogenetics is needed before this 
      technique can be accepted for routine use in the clinic.
FAU - Elis, A
AU  - Elis A
AD  - Department of Medicine and Hematology, Meir Hospital, Kfar Saba, Israel.
FAU - Amiel, A
AU  - Amiel A
FAU - Manor, Y
AU  - Manor Y
FAU - Tangi, I
AU  - Tangi I
FAU - Fejgin, M
AU  - Fejgin M
FAU - Lishner, M
AU  - Lishner M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Thrombocytosis/*genetics
MH  - Trisomy/*diagnosis/genetics
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S016546089600115X [pii]
AID - 10.1016/s0165-4608(96)00115-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Nov;92(1):14-7. doi: 10.1016/s0165-4608(96)00115-x.