PMID- 8956874
OWN - NLM
STAT- MEDLINE
DCOM- 19961230
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 92
IP  - 1
DP  - 1996 Nov
TI  - Selection of probes for fluorescence in situ hybridization analysis by 
      differential display polymerase chain reaction of mRNA from rhabdomyosarcoma.
PG  - 58-65
AB  - Rhabdomyosarcoma (RMS) is a malignancy of skeletal muscle derivation encompassing 
      two major subtypes, embryonal and alveolar, which differ in clinical behavior and 
      genetic markers. Because RMS is a relatively circumscribed tumor system for which 
      the beginnings of a molecular genetic framework are in place, it becomes an ideal 
      model for the application of improved methods of molecular genetic analysis. We 
      have applied the technique known as differential display polymerase chain 
      reaction (DD-PCR) to characterize expression of RNA in rhabdomyosarcoma subtypes. 
      Our studies have shown that DD-PCR generates a characteristic electrophoretic 
      profile that can be used to isolate subtype specific probes for fluorescence in 
      situ hybridization (FISH) analysis. We have isolated two cDNA fragments and 
      obtained clones suitable for FISH mapping to metaphase chromosomes. One probe was 
      mapped to the centromeric region of human chromosome 22 and the other probe to 
      the human chromosome band 6q25-26. This approach demonstrates the utility of 
      DD-PCR as a technique for isolating novel cDNA expressed in tumors and their 
      subsequent use as probes for FISH analysis. As more genes are identified by 
      DD-PCR and their roles in tumorigenesis become defined, they are likely to 
      provide novel targets for future molecular cytogenetic analysis.
FAU - Pienkowska, M
AU  - Pienkowska M
AD  - Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
FAU - Dimitroulakos, J
AU  - Dimitroulakos J
FAU - Zielenska, M
AU  - Zielenska M
FAU - Thorner, P
AU  - Thorner P
FAU - Plavsic, N
AU  - Plavsic N
FAU - Yeger, H
AU  - Yeger H
FAU - Squire, J A
AU  - Squire JA
LA  - eng
SI  - GENBANK/U65438
SI  - GENBANK/U65439
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - DNA Probes/*genetics/isolation & purification
MH  - DNA, Complementary/*genetics/isolation & purification
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Messenger/*analysis
MH  - RNA, Neoplasm/*analysis
MH  - Rhabdomyosarcoma, Alveolar/*genetics
MH  - Rhabdomyosarcoma, Embryonal/*genetics
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - S016546089600132X [pii]
AID - 10.1016/s0165-4608(96)00132-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Nov;92(1):58-65. doi: 10.1016/s0165-4608(96)00132-x.