PMID- 8957184
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20001218
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 17
IP  - 10
DP  - 1996 Oct
TI  - Electrokinetic chromatography in suppressed electroosmotic flow environment: use 
      of a charged cyclodextrin for the separation of enantiomers and geometric 
      isomers.
PG  - 1575-83
AB  - Electrokinetic chromatography (EKC), with negatively-charged cyclodextrins (NCDs) 
      added to the buffer, was conducted in polyacrylamide-coated columns under 
      suppression of electroosmotic flow. The equations of migration and resolution for 
      neutral solutes in this mode of chromatography, which for brevity we term 
      NCD-EKC, are presented. The chiral sulfated cyclodextrin, beta-CD-SBE (IV), used 
      in this study is anionic over the entire pH range accessible to capillary 
      electrophoresis, and the coated columns are stable and provide reproducible 
      performance in the pH range 2.5-8.8. Optimum separation was obtained in the pH 
      range where the solutes are neutral. The incorporation of an alkyl spacer between 
      the sulfate ion and the rim of the cyclodextrin allows an unhindered approach and 
      inclusion of neutral solutes in the cyclodextrin cavity. Solute migration time is 
      inversely proportional to the concentration of the chiral selector. Separation 
      (relative migration time difference) increases with decreasing chiral selector 
      concentration and approaches a maximum, beyond which further decreases in chiral 
      selector concentration result in broad peaks and loss of resolution. A chiral 
      selector concentration of 1% in a 10 mM phosphate buffer produced excellent 
      separation of amino acids and dipeptide enantiomers. In addition to being chiral 
      selectors, cyclodextrins are also known as shape selectors. NCD-EKC is 
      particularly suited for the separation of positional isomers of hydrophobic 
      solutes. The separation of aflatoxin isomers and chlorophenol congeners is 
      presented. In the separation of chlorophenols the more hydrophobic 
      trichlorophenols eluted first and the least hydrophobic, phenol, eluted last.
FAU - Janini, G M
AU  - Janini GM
AD  - SAIC Frederick, NCI-Frederick Cancer Research and Development Center, MD, USA. 
      janini@ncifcrf.gov
FAU - Muschik, G M
AU  - Muschik GM
FAU - Issaq, H J
AU  - Issaq HJ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Amino Acids)
RN  - 0 (Cyclodextrins)
RN  - 0 (Dansyl Compounds)
RN  - 0 (Dipeptides)
RN  - 0 (Sulfates)
SB  - IM
MH  - Amino Acids/chemistry/*isolation & purification
MH  - Chromatography/*methods
MH  - *Cyclodextrins
MH  - Dansyl Compounds
MH  - Dipeptides/chemistry/*isolation & purification
MH  - Electrochemistry
MH  - Electrophoresis, Capillary
MH  - Hydrogen-Ion Concentration
MH  - Mathematics
MH  - Osmosis
MH  - Stereoisomerism
MH  - Sulfates
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1002/elps.1150171014 [doi]
PST - ppublish
SO  - Electrophoresis. 1996 Oct;17(10):1575-83. doi: 10.1002/elps.1150171014.