PMID- 8958063
OWN - NLM
STAT- MEDLINE
DCOM- 19970103
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 89
IP  - 3
DP  - 1996 Nov
TI  - The strain difference in the effect of mercuric chloride on antigen-triggered 
      serotonin release from rat mast cells is not mediated via interferon-gamma.
PG  - 463-7
AB  - Previous work has shown that in vitro exposure of Brown-Norway (BN) rat 
      peritoneal mast cells to mercuric chloride (HgCl2) causes enhancement of 
      subsequent mediator release induced by cross-linking of surface immunoglobulin E 
      (IgE). This enhancing effect is seen significantly less often with peritoneal 
      cells from Lewis rats. In addition HgCl2 has been shown to suppress interferon 
      (IFN)-gamma production by BN but not Lewis splenocytes. Given that IFN-gamma is 
      known to inhibit mediator release by mast cells, we hypothesized that the strain 
      difference in the effect of HgCl2 on mediator release was mediated via a 
      differential effect on IFN-gamma release from T cells in the mixed peritoneal 
      cell population: IFN-gamma release would be suppressed in the case of the BN rat, 
      releasing the mast cells from inhibition and resulting in the enhancing effect of 
      HgCl2. The aim of the study was to test two predictions of this hypothesis. 
      Exposure of BN rat mast cells to IFN-gamma inhibited subsequent antigen-induced 
      mediator release but did not significantly reduce HgCl2-mediated enhancement of 
      this release. Exposure of Lewis rat mast cells to blocking concentrations of 
      anti-IFN-gamma did not reveal any HgCl2-mediated enhancement of mediator release. 
      These observations provide strong evidence against the hypothesis that the 
      differential effects of HgCl2 on BN and Lewis rat mast cells are mediated via 
      IFN-gamma. In addition the results revealed that BN rat mast cells are 
      significantly more sensitive than Lewis rat mast cells to the inhibitory effects 
      of IFN-gamma on antigen-induced mediator release.
FAU - Hodson, D
AU  - Hodson D
AD  - Division of Renal Medicine, St George's Hospital Medical School, London, UK.
FAU - Oliveira, D B
AU  - Oliveira DB
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens)
RN  - 0 (Recombinant Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - 53GH7MZT1R (Mercuric Chloride)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Cell Culture Techniques
MH  - Dose-Response Relationship, Immunologic
MH  - Interferon-gamma/*immunology
MH  - Mast Cells/*drug effects/immunology/metabolism
MH  - Mercuric Chloride/*pharmacology
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred Lew
MH  - Recombinant Proteins
MH  - Serotonin/*metabolism
MH  - Species Specificity
PMC - PMC1456562
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
PMCR- 1997/11/01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PHST- 1997/11/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1996.d01-757.x [doi]
PST - ppublish
SO  - Immunology. 1996 Nov;89(3):463-7. doi: 10.1046/j.1365-2567.1996.d01-757.x.