PMID- 8959349
OWN - NLM
STAT- MEDLINE
DCOM- 19970307
LR  - 20131121
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 7
IP  - 12
DP  - 1996 Dec
TI  - Hepatocyte growth factor/scatter factor-Met signaling induces proliferation, 
      migration, and morphogenesis of pancreatic oval cells.
PG  - 1805-13
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic effector for 
      cells expressing the Met tyrosine kinase receptor. In this investigation, we show 
      that pancreatic oval cells express Met and exhibit a proliferative response to 
      HGF/SF. Additionally, we found that oval cells treated transiently with this 
      factor become "scattered," whereas those exposed to HGF/ SF for extended periods 
      of time form branching tubular structures. These structures possess true lumens, 
      which are lined by cells with ductal features, including apical microvilli, 
      well-developed intercellular junctions, interdigitation of plasma membranes, and 
      abundant cytoplasmic organelles. Interestingly, these ductal structures are 
      formed by HGF/SF-treated cells cultured on plastic dishes in the absence of 
      exogenous extracellular matrix components. Consistent with their ability to form 
      ductal structures in vitro, we found that pancreatic oval cells form ductal 
      adenocarcinomas in nude mice. This study supports the involvement of HGF/SF-Met 
      signaling in the growth, migration, and morphogenesis of pancreatic oval cells 
      and may have important implications for the expansion and morphogenic 
      differentiation of these cells during developmental, regenerative, and neoplastic 
      growth.
FAU - Jeffers, M
AU  - Jeffers M
AD  - Advanced BioScience Laboratories, Inc.-Basic Research Program, National Cancer 
      Institute-Frederick Cancer Research and Development Center, Maryland 21702, USA.
FAU - Rao, M S
AU  - Rao MS
FAU - Rulong, S
AU  - Rulong S
FAU - Reddy, J K
AU  - Reddy JK
FAU - Subbarao, V
AU  - Subbarao V
FAU - Hudson, E
AU  - Hudson E
FAU - Vande Woude, G F
AU  - Vande Woude GF
FAU - Resau, J H
AU  - Resau JH
LA  - eng
GR  - DK37958/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 789U1901C5 (Copper)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adenocarcinoma
MH  - Animals
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - Carcinogenicity Tests
MH  - Cell Division/drug effects
MH  - Cell Movement/*drug effects
MH  - Cell Transplantation
MH  - Cells, Cultured/chemistry/cytology/enzymology
MH  - Copper/deficiency
MH  - DNA/biosynthesis
MH  - Fluorescent Antibody Technique
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Microscopy, Electron
MH  - Morphogenesis/drug effects
MH  - Pancreas/*cytology/ultrastructure
MH  - Rats
MH  - Receptor Protein-Tyrosine Kinases/immunology/*physiology
MH  - Signal Transduction/*drug effects/physiology
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1996 Dec;7(12):1805-13.