PMID- 8961151
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20181130
IS  - 0022-3549 (Print)
IS  - 0022-3549 (Linking)
VI  - 85
IP  - 12
DP  - 1996 Dec
TI  - Development of a single-shot subunit vaccine for HIV-1. 2. Defining optimal 
      autoboost characteristics to maximize the humoral immune response.
PG  - 1346-9
AB  - The design of a single-shot subunit vaccine for HIV-1 with polylactic-coglycolic 
      acid (PLGA) sustained-release technology to effect an autoboost of antigen (MN 
      gp120) at a given time after the primary immunization requires in-depth knowledge 
      about the timing, the duration, and the need for coadjuvant in the autoboost. 
      These questions cannot be answered unambiguously with PLGA microspheres, so we 
      have conducted studies using Alzet minipumps to release antigen at prescribed 
      times to mimic a PLGA autoboost. The results show that a discrete autoboost is 
      preferred over continuous release of antigen, that the time profile of the 
      autoboost (whether pulsatile or a 2-week continuous release) does not affect the 
      booster immune response, and that only antigen is required in the booster 
      immunization (a coadjuvant in the boost does not give higher titers).
FAU - Cleland, J L
AU  - Cleland JL
AD  - Department of Pharmaceutical Research, Genentech, Inc., South San Francisco, CA 
      94080, USA.
FAU - Barron, L
AU  - Barron L
FAU - Berman, P W
AU  - Berman PW
FAU - Daugherty, A
AU  - Daugherty A
FAU - Gregory, T
AU  - Gregory T
FAU - Lim, A
AU  - Lim A
FAU - Vennari, J
AU  - Vennari J
FAU - Wrin, T
AU  - Wrin T
FAU - Powell, M F
AU  - Powell MF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pharm Sci
JT  - Journal of pharmaceutical sciences
JID - 2985195R
RN  - 0 (AIDS Vaccines)
RN  - 0 (HIV Antibodies)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - AIDS Vaccines/*administration & dosage/immunology
MH  - Animals
MH  - Guinea Pigs
MH  - HIV Antibodies/*biosynthesis
MH  - HIV-1/*immunology
MH  - *Lactic Acid
MH  - Microspheres
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/administration & dosage
EDAT- 1996/12/01 00:00
MHDA- 2000/07/19 11:00
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 2000/07/19 11:00 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0022-3549(15)50202-5 [pii]
AID - 10.1021/js960047b [doi]
PST - ppublish
SO  - J Pharm Sci. 1996 Dec;85(12):1346-9. doi: 10.1021/js960047b.