PMID- 8963690
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20190719
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 39
IP  - 4
DP  - 1996
TI  - Tumor necrosis factor-alpha induces pial arteriolar dilation in newborn pigs.
PG  - 241-7
AB  - The present study in piglets was designed to examine cerebrovascular effects of 
      tumor necrosis factor-alpha (TNF alpha) and potential mechanisms involved. 
      Anesthetized new-born pigs with closed cranial windows implanted were used. 
      Effects of nitric oxide synthase (NOS) inhibitors, NG-nitro-L-arginine (L-NNA) 
      and aminoguanidine, and a prostaglandin H synthase inhibitor, indomethacin, on 
      pial arteriolar responses to TNF alpha were determined. In addition, cortical 
      cerebrospinal fluid (CSF) prostanoids (PGE2 and 6-keto-PGF1 alpha) and cyclic 
      nucleotides (cAMP and cGMP) were examined as indices of local cerebral 
      production. Diameters of pial arterioles were recorded every 5 min for 30 min 
      following topical infusion of TNF alpha under the window. CSF was sampled at the 
      end of the 30-min recordings. TNF alpha (10(-8) and 10(-7) M) caused dilation of 
      pial arterioles and increased CSF prostanoids and cyclic nucleotides. 
      Indo-methacin blocked TNF alpha-induced vasodilation and the increase of 
      prostanoids and cAMP, but not of cGMP. L-NNA and amino-guanidine blocked TNF 
      alpha-induced vasodilation. Both inhibitors attenuated TNF alpha-induced 
      prostanoid increase. Aminoguanidine blocked TNF alpha-induced increased cGMP and 
      attenuated the increase in cAMP. These results are consistent with the hypothesis 
      that TNF alpha increases cAMP via prostanoid synthesis. They also suggest that 
      TNF alpha increases cGMP through nitric oxide synthesis, which, in addition, may 
      promote production of prostanoids and, thus, cAMP.
FAU - Shibata, M
AU  - Shibata M
AD  - Department of Physiology and Biophysics, University of Tennessee, Memphis 38163, 
      USA.
FAU - Parfenova, H
AU  - Parfenova H
FAU - Zuckerman, S L
AU  - Zuckerman SL
FAU - Leffler, C W
AU  - Leffler CW
LA  - eng
GR  - P30 CA-21765/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - E0399OZS9N (Cyclic AMP)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cerebral Arteries/*drug effects
MH  - Cerebrovascular Circulation/*drug effects
MH  - Cyclic AMP/metabolism
MH  - Dinoprostone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Swine
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Vasodilation/drug effects
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0361-9230(95)02142-6 [pii]
AID - 10.1016/0361-9230(95)02142-6 [doi]
PST - ppublish
SO  - Brain Res Bull. 1996;39(4):241-7. doi: 10.1016/0361-9230(95)02142-6.