PMID- 8964177
OWN - NLM
STAT- MEDLINE
DCOM- 19961226
LR  - 20041117
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 14
IP  - 2
DP  - 1996 Mar
TI  - Detection of numerical chromosomal abnormalities by fluorescence in situ 
      hybridization of interphase cell nuclei with chromosome-specific probes on 
      archival cytologic samples.
PG  - 178-81
AB  - Fluorescence in situ hybridization (FISH) is rapidly emerging as a tool for 
      analyzing numerical and structural chromosomal abnormalities in both liquid and 
      solid tumors. Most studies make use of fresh samples. To determine the 
      feasibility of detecting numerical chromosomal abnormalities (NCA) by FISH using 
      chromosome-specific probes 8, 12, 17, and X (Vysis, Inc., Downers Grove, IL) on 
      archival cytologic preparations, we studied 23 patient samples, one 
      Papanicolaou-and one Diff-Quik-stained slide per case (46 slides), and two 
      additional unstained slides (fresh ascitic fluids) as controls. Included in this 
      study were nine ascitic fluids (four benign and five malignant), four malignant 
      pleural fluids, three benign bladder washes, and seven malignant fine-needle 
      aspirates (FNA) from various sites. The slides ranged from 1-94 days old. After 
      removal of coverslips using xylene, all slides were destained in a series of 
      alcohol and water washes. Pretreatment of slides with pepsin was followed by the 
      in situ hybridization procedure. Two hundred cells per slide were evaluated for 
      distinct separate signals. Results showed the following: 1) all slides were 
      evaluable except for eight (8/46) which had either too few cells or enough cells 
      but with faint signals, 2) the oldest sample showed distinct signals, 3) 
      previously Diff-Quik-stained slides showed relatively better signals than 
      Papanicolaou-stained slides, 4) samples less than a month old showed relatively 
      better signals, and 5) malignant samples showed various NCA, but not the benign 
      samples. We conclude that FISH on archival cytologic preparation 1) is feasible, 
      although age of the slide is a factor since better signals were seen in those 
      less than a month old, 2) shows better results in previously Diff-Quik-stained 
      slides, and 3) is a tool that can be used in the retrospective study of various 
      liquid and solid neoplasms.
FAU - Cajulis, R S
AU  - Cajulis RS
AD  - Northwestern University, Chicago, IL 60611-3008, USA.
FAU - Frias-Hidvegi, D
AU  - Frias-Hidvegi D
FAU - Yu, G H
AU  - Yu GH
FAU - Eggena, S
AU  - Eggena S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (DNA Probes)
SB  - IM
MH  - Cell Nucleus/genetics/*pathology
MH  - Chromosome Aberrations/*diagnosis/genetics/*pathology
MH  - Chromosome Disorders
MH  - *DNA Probes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Tissue Preservation
EDAT- 1996/03/01 00:00
MHDA- 2000/06/22 10:00
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0339(199603)14:2<178::AID-DC14>3.0.CO;2-J [pii]
AID - 10.1002/(SICI)1097-0339(199603)14:2<178::AID-DC14>3.0.CO;2-J [doi]
PST - ppublish
SO  - Diagn Cytopathol. 1996 Mar;14(2):178-81. doi: 
      10.1002/(SICI)1097-0339(199603)14:2<178::AID-DC14>3.0.CO;2-J.