PMID- 8967406
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 270
IP  - 4 Pt 2
DP  - 1996 Apr
TI  - Effect of high FFA on glycogenolysis in oxidative rat hindlimb muscles during 
      twitch stimulation.
PG  - R766-76
AB  - The effect of elevated free fatty acids (FFA) on carbohydrate (CHO) utilization 
      in the oxidative muscles of the isolated hindlimb was determined using twitch 
      contraction paradigms evoking a wide range of O2 uptakes and glycogenolysis. The 
      hindlimb was perfused with either 0 or 1.8 mM FFA for 10 min at rest and then 
      subjected to 20 min of stimulation at 0.4, 0.7, 1, 2, 3, or 4 Hz. Soleus (Sol), 
      plantaris (Pl), and red gastrocnemius (RG) were sampled after rest perfusion or 
      stimulation. FFA had little effect on glycogenolysis during stimulation, although 
      glycogen sparing occurred with one of the lesser intensity protocols in each 
      muscle (Sol, 0.4 Hz; RG, 0.7 Hz; Pl, 1 Hz). Muscle citrate and acetyl-CoA were 
      elevated in Sol during several stimulation protocols with high FFA, but this 
      effect was inconsistent in Pl and RG. The sparing of glycogen, when it did occur, 
      was generally unrelated to increases in either citrate or acetyl-CoA content. 
      Furthermore, protocols in which citrate or acetyl-CoA were elevated in the 
      presence of elevated FFA did not demonstrate glycogen sparing. Hindlimb lactate 
      efflux at rest was reduced with FFA but unaffected during stimulation. Glucose 
      uptake was unaffected by FFA at rest and during stimulation protocols, except 3 
      Hz. The present study does not support the classically proposed roles of citrate 
      and acetyl-CoA in the FFA-induced downregulation of CHO utilization in 
      electrically stimulated rat skeletal muscle.
FAU - Dyck, D J
AU  - Dyck DJ
AD  - School of Human Biology, University of Guelph, Ontario, Canada.
FAU - Peters, S J
AU  - Peters SJ
FAU - Wendling, P S
AU  - Wendling PS
FAU - Spriet, L L
AU  - Spriet LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 020IUV4N33 (Phosphocreatine)
RN  - 2968PHW8QP (Citric Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 72-89-9 (Acetyl Coenzyme A)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acetyl Coenzyme A/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Citric Acid/metabolism
MH  - Electric Stimulation
MH  - Fatty Acids, Nonesterified/*administration & dosage/pharmacokinetics/pharmacology
MH  - Glucose/metabolism
MH  - Glycogen/*metabolism
MH  - Hindlimb
MH  - Lactic Acid/metabolism
MH  - Male
MH  - *Muscle Contraction
MH  - Muscles/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen Consumption
MH  - Phosphocreatine/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1152/ajpregu.1996.270.4.R766 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Apr;270(4 Pt 2):R766-76. doi: 10.1152/ajpregu.1996.270.4.R766.