PMID- 8968883
OWN - NLM
STAT- MEDLINE
DCOM- 19970319
LR  - 20220408
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 35
IP  - 1
DP  - 1997 Jan
TI  - Detection of adeno-associated virus type 2 sequences in the human genital tract.
PG  - 71-8
AB  - Adeno-associated virus (AAV) is a defective parvovirus with unknown 
      pathogenicity. It requires helper functions for its normal replication in human 
      tissue and therefore is not readily isolated from clinical specimens. We have 
      used the PCR method to examine the following clinical samples for the presence of 
      AAV sequences: (i) 15 nasopharyngeal aspirates from symptomatic patients, (ii) 7 
      swab or fluid specimens from vesicles of patients suspected of having 
      varicella-zoster virus infections, (iii) 21 human papilloma virus-positive 
      genital biopsy specimens, (iv) 61 genital swab specimens from women suspected of 
      having herpes simplex virus (HSV) infection examined either directly or following 
      propagation in tissue culture, (v) 62 samples of first-trimester aborted 
      material, including 38 samples from spontaneous abortions and 24 samples from 
      induced abortions, (vi) 11 samples of chorionic villi taken from women undergoing 
      genetic prenatal diagnosis, and (vii) three lots of cultured human embryonic 
      cells. AAV sequences were detected only in samples taken from the genital tracts 
      of women suspected of having HSV infection and not in any of the other types of 
      samples. Samples from 11 patients were positive for AAV: for 4 patients the 
      original swab sample was positive, for 4 patients the cultured swab sample was 
      positive, and for 3 patients both the original swab samples and the cultures were 
      positive. Five of the 11 patients were infected with HSV. Our study demonstrates 
      the presence of AAV in the female genital tract. However, in contrast to a 
      previous report (E. Tobiasch, M. Rabreau, K. Geletneky, S. Larue-Charlus, F. 
      Severin, N. Becker, and J. R. Schlehofer, J. Med. Virol. 44:215-222, 1994), we 
      did not find solid evidence of its replication in maternal or embryonal tissues 
      from the first trimester of pregnancy. The questions of a potential pathogenic 
      etiology of AAV and the interaction with HSV remain open.
FAU - Friedman-Einat, M
AU  - Friedman-Einat M
AD  - Institute of Hematology, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
FAU - Grossman, Z
AU  - Grossman Z
FAU - Mileguir, F
AU  - Mileguir F
FAU - Smetana, Z
AU  - Smetana Z
FAU - Ashkenazi, M
AU  - Ashkenazi M
FAU - Barkai, G
AU  - Barkai G
FAU - Varsano, N
AU  - Varsano N
FAU - Glick, E
AU  - Glick E
FAU - Mendelson, E
AU  - Mendelson E
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/*analysis
MH  - Dependovirus/*isolation & purification
MH  - Female
MH  - Genitalia, Female/*virology
MH  - Humans
MH  - Parvoviridae Infections/*virology
MH  - Polymerase Chain Reaction
MH  - Pregnancy
PMC - PMC229514
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
PMCR- 1997/01/01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PHST- 1997/01/01 00:00 [pmc-release]
AID - 10.1128/jcm.35.1.71-78.1997 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1997 Jan;35(1):71-8. doi: 10.1128/jcm.35.1.71-78.1997.