PMID- 8969940
OWN - NLM
STAT- MEDLINE
DCOM- 19970313
LR  - 20191024
IS  - 0743-5800 (Print)
IS  - 0743-5800 (Linking)
VI  - 22
IP  - 4
DP  - 1996 Nov
TI  - Adrenal 11 beta-hydroxysteroid dehydrogenase.
PG  - 771-80
AB  - 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyzes the interconversion 
      of cortisol (F) to inactive cortisone (E) in man (corticosterone (B) to 
      11-dehydrocorticosterone (A) in rodents) and plays a crucial role in regulating 
      corticosteroid hormone action. Two isoforms of this enzyme have been 
      characterized; a low affinity NADP(H)-dependent enzyme (11 beta-HSD1) and a high 
      affinity NAD-dependent dehydrogenase (11 beta-HSD2). We have analysed the 
      expression of 11 beta-HSD in the rodent and human adrenal gland and have 
      investigated its role with respect to glucocorticoid-mediated catecholamine 
      biosynthesis. Our studies indicated higher expression of 11 beta-HSD2 mRNA in 
      male versus female intact mouse adrenal. Both 11 beta-HSD isoforms were detected 
      in intact male rat adrenal homogenates. For the 11 beta-HSD1 isoform, 
      NADPH-dependent oxo-reductase activity exceeded that of NADP-dependent 
      dehydrogenase activity (188 versus 98 pmol/mg.protein.hr). In situ hybridisation 
      studies indicated specific localisation of 11 beta-HSD1 mRNA to cells at the 
      corticomedullary junction. 11 beta-HSD2 mRNA was uniformly distributed across the 
      cortex and was low/absent in the medulla. Administration of glycyrrhizic acid in 
      vivo (> 100 mg/kg for 4 days) resulted in inhibition of 11 beta-HSD1 mRNA and 
      activity and a decrease in mRNA levels for the glucocorticoid-dependent enzyme, 
      phenylethanolamine N-methyltransferase, whilst levels of the 
      glucocorticoid-independent enzyme, tyrosine hydroxylase were unchanged. No 11 
      beta-HSD expression was observed in the rat phaeochromocytoma cell line, PC12 
      cells, nor in human normal adrenal gland or phaeochromocytoma specimens. There 
      are marked species and sex differences in the expression of 11 beta-HSD isoforms 
      within the adrenal. The role of 11 beta-HSD within the adrenal gland remains 
      obscure, but at least in the rat, the expression of the reductase enzyme, 11 
      beta-HSD1, to the corticomedullary junction may serve to maintain high medullary 
      glucocorticoid concentrations required for catecholamine biosynthesis.
FAU - Shimojo, M
AU  - Shimojo M
AD  - Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, 
      Edgbaston, England.
FAU - Condon, J
AU  - Condon J
FAU - Whorwood, C B
AU  - Whorwood CB
FAU - Stewart, P M
AU  - Stewart PM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0U46U6E8UK (NAD)
RN  - 53-59-8 (NADP)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Adrenal Gland Neoplasms/enzymology
MH  - Adrenal Glands/*enzymology
MH  - Animals
MH  - Blotting, Northern
MH  - Corticosterone/metabolism
MH  - Female
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/genetics/*metabolism
MH  - In Situ Hybridization
MH  - Isoenzymes/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NAD/pharmacology
MH  - NADP/pharmacology
MH  - Pheochromocytoma/enzymology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1080/07435809609043775 [doi]
PST - ppublish
SO  - Endocr Res. 1996 Nov;22(4):771-80. doi: 10.1080/07435809609043775.