PMID- 8970173
OWN - NLM
STAT- MEDLINE
DCOM- 19970312
LR  - 20200304
IS  - 1073-6085 (Print)
IS  - 1073-6085 (Linking)
VI  - 6
IP  - 2
DP  - 1996 Oct
TI  - Primed in situ labeling (PRINS). A fast method for in situ labeling of nucleic 
      acids.
PG  - 201-11
AB  - PRimed IN Situ labeling (PRINS) is a fast and sensitive alternative to 
      fluorescence in situ hybridization (FISH) for identification of chromosome 
      aberrations. In this article, we present the detailed protocols for detection of 
      repeat sequences using oligonucleotides or fragments of cloned probes as primers 
      for PRINS. We describe a multicolor PRINS procedure for simultaneous 
      visualization of more probes in different colors on a metaphase preparation, and 
      a PRINS-painting procedure, which combines PRINS and chromosome painting. 
      Finally, a protocol for detection of single-copy genes is presented.
FAU - Hindkjaer, J
AU  - Hindkjaer J
AD  - Department of Gynecology and Obsterics, Arhus University Hospital, Denmark.
FAU - Koch, J
AU  - Koch J
FAU - Brandt, C
AU  - Brandt C
FAU - Kolvraa, S
AU  - Kolvraa S
FAU - Bolund, L
AU  - Bolund L
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - Chromosomes, Human
MH  - DNA Primers
MH  - DNA Probes
MH  - DNA, Satellite
MH  - Female
MH  - Genetic Techniques
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acids/*chemistry
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - 10.1007/BF02740774 [doi]
PST - ppublish
SO  - Mol Biotechnol. 1996 Oct;6(2):201-11. doi: 10.1007/BF02740774.