PMID- 8970622
OWN - NLM
STAT- MEDLINE
DCOM- 19970115
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 62
IP  - 11
DP  - 1996 Dec 15
TI  - Docosahexaenoic and eicosapentaenoic acids inhibit in vitro human 
      lymphocyte-endothelial cell adhesion.
PG  - 1649-57
AB  - Dietary supplementation with fish oil, which contains high amounts of long chain 
      omega 3 ((n-3)) polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic 
      acid (DHA) and eicosapentaenoic acid (EPA), has recently been shown to have 
      protective and ameliorative effects on diseases characterized by chronic 
      inflammatory reactions. Interactions between vascular endothelium, mononuclear 
      cells, and cytokines are crucial steps in the course of inflammatory processes 
      such as chronic graft rejection. We therefore studied the effects of DHA and EPA 
      on both the adhesion of peripheral blood lymphocytes (PBL) to human endothelial 
      cells (EC) in culture and the expression of EC-adhesion molecules and their 
      counterreceptors on PBL. The addition of DHA or EPA to the adhesion assay 
      significantly decreased the adhesion of PBL to untreated EC and tumor necrosis 
      factor-alpha (TNF alpha)-, interleukin (IL) 4-, and lipopolysaccharide-stimulated 
      EC. When EC were pretreated with (n-3) PUFAs for 18 hr, washed, and then 
      stimulated by TNF alpha, IL-4, or lipopolysaccharide, PBL adhesion was also 
      significantly reduced compared with controls. We also showed that PBL 
      preincubated with DHA or EPA, and then washed and chromium radiolabeled, still 
      exhibited an adhesion inhibition to TNF alpha- and IL-4-treated EC as well as 
      untreated EC. Cytofluorometry and immunoenzymatic analyses indicated that 
      pretreatment of EC with (n-3) PUFAs before their activation significantly reduced 
      the EC-induced expression of vascular cell adhesion molecule 1, whereas the level 
      of expression of intercellular adhesion molecule 1 and E-selectin was not 
      modified. Furthermore, we showed that incubation of PBL with DHA or EPA 
      moderately reduced the level of cell surface expression of L-selectin and 
      leukocyte function-associated antigen 1, but not of very late antigen 4. In all 
      cases, the inhibitory effect of (n-3) PUFAs was specific and dose dependent. In 
      addition, DHA seems to be a more potent inhibitor than EPA, but the two compounds 
      in association had an additive effect. Regardless of the mode of action, this 
      inhibitory effect may explain the protective and ameliorative effects of (n-3) 
      PUFAs on diseases involving chronic inflammatory reaction.
FAU - Khalfoun, B
AU  - Khalfoun B
AD  - Groupe Interactions Hote-Greffon, Faculte de Medecine, Tours, France.
FAU - Thibault, G
AU  - Thibault G
FAU - Bardos, P
AU  - Bardos P
FAU - Lebranchu, Y
AU  - Lebranchu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
SB  - IM
MH  - Cell Adhesion/*drug effects
MH  - Cell Adhesion Molecules/biosynthesis
MH  - Cell Survival/drug effects
MH  - Dietary Fats, Unsaturated/pharmacology
MH  - Docosahexaenoic Acids/*pharmacology
MH  - Eicosapentaenoic Acid/*pharmacology
MH  - Endothelium, Vascular/*cytology
MH  - Fatty Acids, Unsaturated/administration & dosage
MH  - Humans
MH  - Lymphocytes/*cytology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1996/12/15 00:00
MHDA- 1996/12/15 00:01
CRDT- 1996/12/15 00:00
PHST- 1996/12/15 00:00 [pubmed]
PHST- 1996/12/15 00:01 [medline]
PHST- 1996/12/15 00:00 [entrez]
AID - 10.1097/00007890-199612150-00020 [doi]
PST - ppublish
SO  - Transplantation. 1996 Dec 15;62(11):1649-57. doi: 
      10.1097/00007890-199612150-00020.