PMID- 8972635
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190818
IS  - 0031-8655 (Print)
IS  - 0031-8655 (Linking)
VI  - 64
IP  - 6
DP  - 1996 Dec
TI  - Induction of cyclobutane pyrimidine dimer photolyase in cultured fish cells by 
      UVA and blue light.
PG  - 943-8
AB  - The cyclobutane pyrimidine dimer (CPD) photolyase in fish cells is known to be 
      regulated by environmental factors, such as light, hydrogen peroxide and growth 
      inhibition. The induction of CPD photolyase by light in cultured goldfish cells 
      was dependent on the wavelength of the light, and UVA and blue light had high 
      inductive activity. The spectrum for CPD photolyase activity was different from 
      that for the induction. Treatment with blue or yellow light for a short time, 
      which did not induce any CPD photolyase, induced high CPD photolyase activity in 
      the presence of the photosensitizers, TPPS (monosulfonated meso-tetraphenyl 
      porphine) and ALPS (aluminum phthalocyanine tetrasulfonate), respectively. These 
      results suggest that the induction of CPD photolyase might be triggered by active 
      oxygen produced by light and cellular photosensitizers. We also found that 
      immediately after treatment with UVA, blue light or a photosensitizer in 
      combination with light, cellular attachment to the substratum was enhanced, as 
      was the CPD photolyase activity. Pretreatment with a flavonoid, quercetin, 
      inhibited both photoinduction of CPD photolyase and enhancement of cellular 
      attachment. Vitamin E inhibited only photoinduction of CPD photolyase activity. 
      Treatment with H7, a strong inhibitor for protein kinase C, after light treatment 
      inhibited photoinduction of CPD photolyase activity, but an analogue of H7, 
      Ha1004, which is a weak inhibitor of protein kinase C, did not have such an 
      effect.
FAU - Mitani, H
AU  - Mitani H
AD  - Department of Biological Sciences, University of Tokyo, Japan. 
      mitani@biol.s.u-tokyo.ac.jp
FAU - Uchida, N
AU  - Uchida N
FAU - Shima, A
AU  - Shima A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - EC 4.1.99.3 (Deoxyribodipyrimidine Photo-Lyase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Deoxyribodipyrimidine Photo-Lyase/*biosynthesis
MH  - Enzyme Induction
MH  - Goldfish
MH  - *Light
MH  - *Ultraviolet Rays
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1111/j.1751-1097.1996.tb01859.x [doi]
PST - ppublish
SO  - Photochem Photobiol. 1996 Dec;64(6):943-8. doi: 
      10.1111/j.1751-1097.1996.tb01859.x.