PMID- 8975874
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 60
IP  - 6
DP  - 1996 Dec
TI  - The use of chimeric human Fc(epsilon) receptor I to redirect cytotoxic T 
      lymphocytes to tumors.
PG  - 721-8
AB  - Chimeric receptors that redirect effector cell function to tumor cells or 
      virus-infected cells have received much attention. Given the high affinity of 
      Fc(epsilon)RI for immunoglobulin E (IgE) and low serum IgE levels, redirection of 
      effector cells using Fc(epsilon) receptor may provide a novel, versatile, and 
      effective anti-tumor strategy. We have used a mouse perforin 5'-promoter to 
      express a single-chain human Fc(epsilon) receptor in the mouse cytotoxic T 
      lymphocyte cell line, CTLL-R8. Upon ligation of the chimeric Fc(epsilon) 
      receptors by IgE, a signal for effector function is transmitted via the 
      intracellular domain of CD3zeta. Selection in G418-containing medium produced 
      CTLLR8 transfectant clones that: (1) expressed chimeric Fc(epsilon) receptor as 
      determined by flow cytometry; (2) bound human IgE antibodies with high affinity 
      as determined by Scatchard analysis; (3) specifically rosetted IgE-coated SRBC; 
      (4) lysed target cells in IgE-mediated ADCC and reverse ADCC assays; and (5) 
      retarded tumor growth in a Winn assay. Therefore these chimeric Fc(epsilon) 
      receptors can effectively redirect cytotoxicity to tumor cells. Future efforts 
      will assess the versatility and efficacy of these IgE-binding chimeric receptors 
      to redirect killer cell function in animal tumor models.
FAU - Kershaw, M H
AU  - Kershaw MH
AD  - Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.
FAU - Darcy, P K
AU  - Darcy PK
FAU - Trapani, J A
AU  - Trapani JA
FAU - Smyth, M J
AU  - Smyth MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CD3 Complex)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 126465-35-8 (Perforin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibody-Dependent Cell Cytotoxicity
MH  - CD3 Complex/chemistry
MH  - Cytotoxicity, Immunologic
MH  - Dose-Response Relationship, Immunologic
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Immunotherapy
MH  - Membrane Glycoproteins/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasms, Experimental/*therapy
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins
MH  - Receptors, IgE/*chemistry/immunology
MH  - Recombinant Fusion Proteins
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transfection
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1002/jlb.60.6.721 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Dec;60(6):721-8. doi: 10.1002/jlb.60.6.721.