PMID- 8975884
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 60
IP  - 6
DP  - 1996 Dec
TI  - Monocyte chemoattractant protein-1: receptor interactions and calcium signaling 
      mechanisms.
PG  - 793-803
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a member of the Cys-Cys chemokine 
      family. Two related MCP-1 receptors have been identified (CC-CKR2A and CC-CKR2B), 
      although the precise kinetics of ligand binding and calcium signaling of these 
      receptors has yet to be investigated. To examine this more closely, the human 
      MCP-1 receptors were cloned and expressed in Chinese hamster ovary (CHO) cells. 
      Membranes prepared from cells expressing CC-CKR2B bind MCP-1 selectively and with 
      high affinity (Kd = 120 pM). MCP-1 stimulation of recombinant CHO cells 
      expressing CC-CKR2B induces a rapid increase in intracellular Ca2+ through both 
      receptor-operated Ca2+ channels and mobilization of Ca2+ from intracellular 
      stores, and leads to a rapid temperature-dependent internalization of the 
      ligand/receptor complexes. In contrast, recombinant CHO cells expressing 
      CC-CKR2A, and membranes prepared from these cells, fail to bind detectable levels 
      of MCP-1. However, MCP-1 stimulation of cells expressing CC-CKR2A induces a small 
      but significant increase in intracellular Ca2+. Repeated stimulation of these 
      cells with MCP-1 leads to a potentiation of the response to a level comparable to 
      that seen in cells expressing CC-CKR2B. These observations suggest that the 
      levels of cell surface CC-CKR2A are controlled by novel mechanisms.
FAU - Needham, M
AU  - Needham M
AD  - Cardiovascular & Musculoskeletal Research Department, ZENECA Pharmaceuticals, 
      Mereside, Cheshire, United Kingdom.
FAU - Sturgess, N
AU  - Sturgess N
FAU - Cerillo, G
AU  - Cerillo G
FAU - Green, I
AU  - Green I
FAU - Warburton, H
AU  - Warburton H
FAU - Wilson, R
AU  - Wilson R
FAU - Martin, L
AU  - Martin L
FAU - Barratt, D
AU  - Barratt D
FAU - Anderson, M
AU  - Anderson M
FAU - Reilly, C
AU  - Reilly C
FAU - Hollis, M
AU  - Hollis M
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Recombinant Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Calcium/*physiology
MH  - Cell Membrane/metabolism
MH  - Chemokine CCL2/*physiology
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Endocytosis
MH  - Humans
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/chemistry/*physiology
MH  - Recombinant Proteins
MH  - Signal Transduction
MH  - Time Factors
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1002/jlb.60.6.793 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Dec;60(6):793-803. doi: 10.1002/jlb.60.6.793.