PMID- 8976189
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 184
IP  - 6
DP  - 1996 Dec 1
TI  - Germinal center formation and local immunoglobulin E (IgE) production in the lung 
      after an airway antigenic challenge.
PG  - 2353-60
AB  - Airway inflammation plays a central role in the pathogenesis of asthma. However, 
      the precise contribution of all cell types in the development and maintenance of 
      airway hyperreactivity and histopathology during allergic inflammation remains 
      unclear. After sensitization of mice in the periphery, challenge by multiple 
      intratracheal (i.t.) instillations of ovalbumin (OVA) results in eosinophilia, 
      mononuclear cell infiltration, and airway epithelial changes analogous to that 
      seen in asthma (Blyth, D.I., M.S. Pedrick, T.J. Savage, E.M. Hessel, and D. 
      Fattah. 1996. Am. J. Respir. Cell Mol. Biol. 14:425-438). To investigate further 
      the nature of the cellular infiltrate, lungs from OVA-versus saline-treated mice 
      were processed for histology and immunohistochemistry. One of the most striking 
      features observed was the formation of germinal centers within the parenchyma of 
      the inflamed lungs. In addition, follicular dendritic cells (FDCs) bearing OVA on 
      their plasma membranes appeared and, adjacent to these sites, OVA-specific IgG1-, 
      IgE-, and IgA-producing plasma cells emerged. To confirm that antigen-specific 
      immunoglobulins (Ig) were being produced within the parenchyma, plasma cell 
      number and antibody production were quantitated in vitro after isolation of cells 
      from the lung. These assays confirmed that the isotypes observed in situ were a 
      secreted product. As IgE-dependent mechanisms have been implicated as being 
      central to the pathogenesis of bronchial asthma, airway hyperresponsiveness was 
      evaluated. The mice undergoing lung inflammation were hyperresponsive, while the 
      control group remained at baseline. These data demonstrate that antigen-driven 
      differentiation of B cells via induction of an FDC network and germinal centers 
      occurs in the parenchyma of inflamed lungs. These germinal centers would then 
      provide a local source of IgE-secreting plasma cells that contribute to the 
      release of factors mediating inflammatory processes in the lung.
FAU - Chvatchko, Y
AU  - Chvatchko Y
AD  - Department of Immunology, Geneva Biomedical Research Institute, Glaxo Wellcome 
      Research and Development S.A., Switzerland.
FAU - Kosco-Vilbois, M H
AU  - Kosco-Vilbois MH
FAU - Herren, S
AU  - Herren S
FAU - Lefort, J
AU  - Lefort J
FAU - Bonnefoy, J Y
AU  - Bonnefoy JY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Female
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Inflammation
MH  - Instillation, Drug
MH  - Lung/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mucous Membrane/immunology
MH  - Ovalbumin/administration & dosage/*immunology
MH  - Plasma Cells/immunology
MH  - Trachea/*immunology
PMC - PMC2196373
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
PMCR- 1997/06/01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PHST- 1997/06/01 00:00 [pmc-release]
AID - 10.1084/jem.184.6.2353 [doi]
PST - ppublish
SO  - J Exp Med. 1996 Dec 1;184(6):2353-60. doi: 10.1084/jem.184.6.2353.