PMID- 8976370
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 92
IP  - 2
DP  - 1996 Dec
TI  - Tetrasomy 8 detected by interphase cytogenetics in a child with acute lymphocytic 
      leukemia.
PG  - 135-40
AB  - Tetrasomy 8 is a rare clonal anomaly in human acute leukemia. Here we present a 
      case of a 7-year-old boy with acute lymphoblastic leukemia (ALL) displaying a 
      tetrasomy 8 clone that could not be detected by conventional cytogenetics. In 
      this study, bone marrow and peripheral blood cells were collected at five 
      different diagnostic stages and analyzed by double targeted fluorescence in situ 
      hybridization (FISH) with centromeric DNA probes for chromosomes 7, 8, 9, and 12. 
      FISH analysis revealed a significant increase in tetrasomy 8 frequency, but not 
      in other chromosomes examined. A smaller increase in trisomy 8 was also detected. 
      At one stage over 60% of the cells were hyperdiploid with 40% being tetrasomic. 
      The size of the tetrasomic clone changed during the course of the disease. The 
      hyperdiploid frequencies of chromosome 8 detected by interphase FISH analysis in 
      bone marrow and peripheral blood were similar. Our findings indicate the utility 
      of FISH analysis in cytogenetic monitoring of leukemia patients and further show 
      that tetrasomy 8 may play a specific role in a subtype of ALL.
FAU - Shao, J
AU  - Shao J
AD  - School of Public Health, University of California, Berkeley 94720-7360, USA.
FAU - Zhang, L
AU  - Zhang L
FAU - Semenza, J C
AU  - Semenza JC
FAU - Beach, B
AU  - Beach B
FAU - Smith, M T
AU  - Smith MT
LA  - eng
GR  - P42-ES04705/ES/NIEHS NIH HHS/United States
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Aneuploidy
MH  - Bone Marrow/pathology
MH  - Cells, Cultured
MH  - Child
MH  - Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 8
MH  - Chromosomes, Human, Pair 9
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Neoplasm Staging
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood/drug 
      therapy/*genetics/pathology
MH  - Time Factors
MH  - Trisomy
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0165460896001811 [pii]
AID - 10.1016/s0165-4608(96)00181-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Dec;92(2):135-40. doi: 
      10.1016/s0165-4608(96)00181-1.