PMID- 8976763
OWN - NLM
STAT- MEDLINE
DCOM- 19970109
LR  - 20190719
IS  - 0007-1331 (Print)
IS  - 0007-1331 (Linking)
VI  - 78
IP  - 5
DP  - 1996 Nov
TI  - Genomic heterogeneity in bladder cancer as detected by fluorescence in situ 
      hybridization.
PG  - 699-703
AB  - OBJECTIVE: To investigate the relationship between genomic heterogeneity and 
      tumour grade, stage and DNA content in 30 transitional cell carcinomas (TCCs) of 
      the urinary bladder. MATERIALS AND METHODS: Tissue specimens from 30 patients (25 
      men and five women) with newly diagnosed TCC of the urinary bladder were examined 
      for genomic heterogeneity using fluorescence in situ hybridization (FISH) with 
      chromosome-specific DNA probes; the copy number of pericentromeric sequences on 
      chromosomes 7, 9 and 17 was detected within interphase nuclei in contact 
      preparations from the tumour specimens. RESULTS: The aneusomy of chromosomes 7, 9 
      and 17 was significantly higher in aneuploid than in diploid tumours (P < 0.001). 
      Tumour grade and stage were strongly associated with aneusomy for chromosome 17 
      (P < 0.01, P < 0.001, respectively). The aneusomy of chromosomes 7 and 9 were 
      significantly correlated with increasing tumour stage (P < 0.001), but not with 
      tumour grade. CONCLUSION: These results suggest that the measurement of aneusomy 
      using FISH, especially for chromosome 17, in bladder cancer may offer a new 
      objective and quantitative assay of the biological potential of individual 
      tumours.
FAU - Yokogi, H
AU  - Yokogi H
AD  - Department of Urology, Shimane Medical University, Izumo, Japan.
FAU - Wada, Y
AU  - Wada Y
FAU - Moriyama-Gonda, N
AU  - Moriyama-Gonda N
FAU - Igawa, M
AU  - Igawa M
FAU - Ishibe, T
AU  - Ishibe T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Urol
JT  - British journal of urology
JID - 15740090R
RN  - 0 (DNA Probes)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Carcinoma, Transitional Cell/*genetics/pathology
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 7
MH  - *Chromosomes, Human, Pair 9
MH  - DNA Probes
MH  - *Diploidy
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1046/j.1464-410x.1996.01786.x [doi]
PST - ppublish
SO  - Br J Urol. 1996 Nov;78(5):699-703. doi: 10.1046/j.1464-410x.1996.01786.x.