PMID- 8977213
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 158
IP  - 1
DP  - 1997 Jan 1
TI  - Monocyte chemoattractant protein-1 is sufficient for the chemotaxis of monocytes 
      and lymphocytes in transgenic mice but requires an additional stimulus for 
      inflammatory activation.
PG  - 376-83
AB  - Monocyte chemoattractant protein-1 (MCP-1), a chemotactic cytokine, acts in vitro 
      as a chemotactic and activating factor for multiple types of leukocytes. To 
      determine the chemotactic and activating effects of MCP-1 in vivo, we constructed 
      transgenic mice that express human MCP-1 in type II alveolar epithelial cells and 
      secrete it into the bronchoalveolar space. We found that MCP-1 overexpression led 
      to a marked increase in the numbers of both monocytes and lymphocytes that could 
      be recovered by bronchoalveolar lavage. This accumulation of mononuclear 
      leukocytes could be reversed by the administration of an MCP-1-blocking Ab. In 
      spite of its chemotactic effect, MCP-1 expression did not cause the inflammatory 
      activation of accumulated leukocytes. Lungs of MCP-1 transgenic mice also showed 
      no morphologic evidence of inflammation. However, MCP-1 mice had an increased 
      sensitivity to other inflammatory stimuli. MCP-1 mice treated with either i.p. 
      LPS or i.v. yeast wall glucan developed consolidated pulmonary infiltrates 
      consisting predominantly of macrophages. Nontransgenic mice developed no such 
      infiltrates. These results demonstrate that MCP-1 is chemotactic for monocytes 
      and lymphocytes in vivo and that MCP-1 expression alone does not cause 
      inflammatory activation of cells, but leads to an enhanced inflammatory response 
      upon treatment with other stimuli.
FAU - Gunn, M D
AU  - Gunn MD
AD  - Daiichi Research Center, Cardiovascular Research Institute, University of 
      California at San Francisco 94143, USA.
FAU - Nelken, N A
AU  - Nelken NA
FAU - Liao, X
AU  - Liao X
FAU - Williams, L T
AU  - Williams LT
LA  - eng
GR  - K11 HL02388/HL/NHLBI NIH HHS/United States
GR  - P01 HL43821/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glucans)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*pharmacology
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Drug Synergism
MH  - Epithelium/metabolism
MH  - Glucans/pharmacology
MH  - Humans
MH  - Inflammation/*immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation/*drug effects
MH  - Lymphocytes/*drug effects
MH  - Macrophage Activation/*drug effects
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/*drug effects
MH  - Pulmonary Alveoli/metabolism
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1997 Jan 1;158(1):376-83.