PMID- 8978459
OWN - NLM
STAT- MEDLINE
DCOM- 19970317
LR  - 20181003
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 63
IP  - 4
DP  - 1996 Dec 15
TI  - Fluid shear stress induces actin polymerization in human neutrophils.
PG  - 432-41
AB  - We have previously reported that a physiological range of shear stress induces 
      neutrophil homotypic aggregation mediated by lymphocyte function-associated 
      antigen-1 (LFA-1) and intercellular adhesion molecule-3 (ICAM-3) interactions. To 
      further characterized the homotypic aggregation, actin polymerization was 
      investigated in neutrophils stimulated by shear stress in comparison with 
      formyl-methionyl-leucyl-phenylalanine (fMLP). In fMLP-stimulated neutrophils, 
      actin polymerization was localized in the pseudopods, and this reaction was not 
      mediated by a cytosolic level of Ca2+. In contrast to fMLP stimulation, the actin 
      polymerization induced by shear stress in a cone-plate viscometer was localized 
      in cell-cell contact regions, and this polymerization required the increase of 
      intracellular Ca2+. This shear stress-induced actin polymerization was not 
      observed when neutrophils were pretreated with anti-LFA-1 or anti-ICAM-3 
      antibody. In conclusion, LFA-1 and ICAM-3 interaction mediated by the increase of 
      [Ca2+]i generated the intercellular signal in order to accumulate F-actin in the 
      cell-cell contact regions.
FAU - Okuyama, M
AU  - Okuyama M
AD  - Department of Surgery II, Osaka University Medical School, Japan.
FAU - Ohta, Y
AU  - Ohta Y
FAU - Kambayashi, J
AU  - Kambayashi J
FAU - Monden, M
AU  - Monden M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Actins)
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (ICAM3 protein, human)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 0 (Polymers)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/drug effects/*metabolism
MH  - Antibodies, Blocking/immunology
MH  - *Antigens, CD
MH  - *Antigens, Differentiation
MH  - Calcium/metabolism
MH  - Cell Adhesion Molecules/immunology
MH  - Cytosol/metabolism
MH  - Humans
MH  - Lymphocyte Function-Associated Antigen-1/immunology
MH  - Microscopy, Confocal
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation
MH  - Neutrophils/drug effects/*metabolism/physiology
MH  - Polymers/*metabolism
MH  - Pseudopodia/metabolism
MH  - Signal Transduction
MH  - Stress, Mechanical
EDAT- 1996/12/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/12/15 00:00
PHST- 1996/12/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/12/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-4644(19961215)63:4<432::AID-JCB5>3.0.CO;2-U [pii]
AID - 10.1002/(SICI)1097-4644(19961215)63:4%3C432::AID-JCB5%3E3.0.CO;2-U [doi]
PST - ppublish
SO  - J Cell Biochem. 1996 Dec 15;63(4):432-41. doi: 
      10.1002/(SICI)1097-4644(19961215)63:4%3C432::AID-JCB5%3E3.0.CO;2-U.