PMID- 8978657
OWN - NLM
STAT- MEDLINE
DCOM- 19970328
LR  - 20190909
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 34
IP  - 1
DP  - 1997 Jan
TI  - Retinal pigment epithelium cells cultured on synthetic biodegradable polymers.
PG  - 87-93
AB  - Alterations in the normal structure or functions of retinal pigment epithelium 
      (RPE) can result in a number of ocular diseases. Implantation of RPE cells 
      cultured on thin, biodegradable polymer films may provide a means of 
      transplanting an organized sheet of RPE cells with distinct apical/basal 
      characteristics for the restoration of normal RPE function. We have investigated 
      the interactions of human RPE cells with different biodegradable polymer films to 
      assess their suitability as substrates for RPE culture. Four biodegradable 
      polymers were used: low molecular weight (MW) 50:50 poly(DL-lactic-co-glycolic 
      acid) (PLGA); high MW 50:50 PLGA; 75:25 PLGA; and poly(L-lactic acid) (PLLA). 
      Polymer film substrates were manufactured using a solvent casting technique. 
      Human fetal RPE cells (10-16 weeks gestational) were plated on the polymer 
      substrates and the cultures assessed with respect to cell attachment and 
      proliferation. Histological and immunohistochemical studies were performed on the 
      cells after 8 days in culture. RPE cells attached to all the polymers studied 
      after 8 h in culture. After 8 h, 80.2 +/- 9.5% and 82.3 +/- 7.9% of the plated 
      cells were attached to substrates of high MW 50:50 PLGA and 75:25 PLGA, 
      respectively. The cells proliferated on all substrates, and there was about a 
      threefold increase in cell number over the 8-day culture period on all the 
      polymers studied. Immunohistochemistry after 8 days in culture demonstrated RPE 
      cells labeled with a distinct reaction product for cytokeratin in the cell 
      cytoplasm. All the polymers studied were suitable for RPE culture; however, high 
      MW 50:50 PLGA and 75:25 PLGA proved to be the best in terms of manufacturing 
      properties, cell attachment, and proliferation. These polymers can provide a 
      suitable substrate for RPE cell culture and hold promise for the subretinal 
      implantation of organized sheets of RPE cells.
FAU - Giordano, G G
AU  - Giordano GG
AD  - Department of Ophthalmology, University of Texas Health Science Center, Houston 
      77030, USA.
FAU - Thomson, R C
AU  - Thomson RC
FAU - Ishaug, S L
AU  - Ishaug SL
FAU - Mikos, A G
AU  - Mikos AG
FAU - Cumber, S
AU  - Cumber S
FAU - Garcia, C A
AU  - Garcia CA
FAU - Lahiri-Munir, D
AU  - Lahiri-Munir D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
SB  - IM
MH  - Biodegradation, Environmental
MH  - Cell Adhesion/physiology
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*cytology
MH  - Humans
MH  - Immunohistochemistry
MH  - *Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - Molecular Weight
MH  - Pigment Epithelium of Eye/*cytology
OTO - NASA
OT  - Non-programmatic
EDAT- 1997/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-4636(199701)34:1<87::AID-JBM12>3.0.CO;2-M [pii]
AID - 10.1002/(sici)1097-4636(199701)34:1<87::aid-jbm12>3.0.co;2-m [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1997 Jan;34(1):87-93. doi: 
      10.1002/(sici)1097-4636(199701)34:1<87::aid-jbm12>3.0.co;2-m.