PMID- 8980621
OWN - NLM
STAT- MEDLINE
DCOM- 19970113
LR  - 20101118
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 104
IP  - 10
DP  - 1996 Oct
TI  - Local and systemic antibody levels against protein D of Haemophilus influenzae 
      following immunization and infection in rats.
PG  - 709-17
AB  - Outer membrane proteins of Haemophilus influenzae are considered as possible 
      vaccine candidates against non-typable H. influenzae (NTHi), a major cause of 
      respiratory infections. Here, we study local and systemic antibody responses to 
      protein D, a well-conserved 42-kDa membrane protein, following local and systemic 
      immunization, and experimental acute otitis media (AOM) with NTHi and H. 
      influenzae type b (Hib) in rats. Animals that were challenged and rechallenged in 
      the middle ear with Hib strain Minn A or NTHi strain 1161 developed IgG and IgA 
      antibodies in serum but not in middle ear lavage (MEL) material or saliva. In 
      contrast, following per oral immunization with NTHi strain 772 and Escherichia 
      coli JM83 (pHIC348) (containing protein D gene) and, to a lesser degree after 
      intranasal inoculation of NTHi strain 772, high saliva IgA antibodies to protein 
      D developed, but there was no rise in antibodies to protein D in the MEL material 
      or the sera of these animals. These results show that protein D can elicit 
      different systemic and local antibody responses depending on the site of delivery 
      and the form of administration. Furthermore, experimental AOM with NTHi and Hib 
      induces systemic IgG and IgA antibodies to protein D but fails to induce a 
      mucosal immune response.
FAU - Akkoyunlu, M
AU  - Akkoyunlu M
AD  - Department of Medical Microbiology, University of Lund, Malmo, Sweden.
FAU - Forsgren, A
AU  - Forsgren A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lipoproteins)
RN  - 0 (Vaccines, Synthetic)
RN  - EC 3.1.4.46 (glpQ protein, Haemophilus influenzae)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood/*immunology
MH  - Bacterial Proteins/*immunology
MH  - Carrier Proteins/*immunology
MH  - Ear, Middle/immunology
MH  - Escherichia coli
MH  - Haemophilus Vaccines/administration & dosage/*immunology
MH  - Haemophilus influenzae/*immunology
MH  - Humans
MH  - Immunity, Mucosal
MH  - Immunoglobulin A/blood/immunology
MH  - *Immunoglobulin D
MH  - Immunoglobulin G/blood/immunology
MH  - Lipoproteins/*immunology
MH  - Otitis Media/immunology/microbiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Saliva/immunology
MH  - Therapeutic Irrigation
MH  - Vaccination
MH  - Vaccines, Synthetic/immunology
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
PST - ppublish
SO  - APMIS. 1996 Oct;104(10):709-17.