PMID- 8980663
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 70
IP  - 6
DP  - 1996 Dec
TI  - Comparison between fluorescence in situ hybridization and conventional 
      cytogenetics for dicentric scoring: a first-step validation for the use of FISH 
      in biological dosimetry.
PG  - 665-9
AB  - In this study the suitability of fluorescence in situ hybridization (FISH) for 
      dicentric detection using a commercially available alpha-satellite probe (Oncor) 
      to label centromeres was compared with the conventional technique for the 
      detection of unstable aberrations. A standard FISH protocol was applied for 
      centromere labelling. Dose-response curves using blood samples irradiated in 
      vitro with gamma-rays (60Co) at a dose-rate of 0.1 Gy/min were established using 
      both techniques and compared. No statistical difference was observed between 
      either method. The FISH technique thus allows a correct detection of unstable 
      aberrations when an alpha-satellite DNA probe is used.
FAU - Roy, L
AU  - Roy L
AD  - Institut de Protection et de Surete Nucleaire, Departement de Protection de la 
      sante de l'Homme, Fontenay-aux-Roses, France.
FAU - Sorokine-Durm, I
AU  - Sorokine-Durm I
FAU - Voisin, P
AU  - Voisin P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Centromere/*genetics
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Poisson Distribution
MH  - Ring Chromosomes
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1080/095530096144545 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1996 Dec;70(6):665-9. doi: 10.1080/095530096144545.