PMID- 8982070
OWN - NLM
STAT- MEDLINE
DCOM- 19970122
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 182
IP  - 1-2
DP  - 1996 Dec 5
TI  - Reexamining interaction of the SH2 domains of SYP and GAP with insulin and IGF-1 
      receptors in the two-hybrid system.
PG  - 77-80
AB  - Direct interaction of effector proteins such as the p85 regulatory subunit of 
      phosphatidylinositol 3-kinase (PI 3-kinase), SYP (SH2-domain-containing tyrosine 
      phosphatase) and GAP (Ras-GTPase activating protein) with the insulin receptor 
      (IR) and insulin-like growth factor-1 (IGF-1) type 1 receptor (IGF-1R) has been 
      reported in some studies. Interaction of SYP and GAP with IR and IGF-1R was 
      re-investigated here in the two-hybrid system by assessing his3/lacZ activation 
      in S. cerevisiae. The experiments were performed with the cytoplasmic beta domain 
      of IR and IGF-1R and various SH2-subdomains of SYP and GAP. None of the 
      subdomains of SYP and GAP tested were able to activate his3/lacZ, whereas these 
      reporter genes were strongly activated when p85 was used as we have recently 
      shown. Thus, interaction of SYP and GAP with IR and IGF-1R, if any, would be weak 
      and/or transient as compared to that of p85.
FAU - Lamothe, B
AU  - Lamothe B
AD  - Institut Cochin de Genetique Moleculaire, INSERM U257, Paris, France.
FAU - Bucchini, D
AU  - Bucchini D
FAU - Jami, J
AU  - Jami J
FAU - Joshi, R L
AU  - Joshi RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (GAL4 protein, S cerevisiae)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LexA protein, Bacteria)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ras GTPase-Activating Proteins)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
RN  - EC 2.7.10.1 (Receptor, Insulin)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 6)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (SH2 Domain-Containing Protein Tyrosine Phosphatases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Bacterial Proteins/genetics/metabolism
MH  - Cell Division/genetics
MH  - DNA Primers
MH  - DNA-Binding Proteins
MH  - Fungal Proteins/genetics/metabolism
MH  - GTPase-Activating Proteins
MH  - Genes, Reporter/genetics
MH  - Intracellular Signaling Peptides and Proteins
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 1
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 11
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 6
MH  - Protein Tyrosine Phosphatases/*metabolism
MH  - Proteins/*metabolism
MH  - Receptor, IGF Type 1/chemistry/*metabolism
MH  - Receptor, Insulin/chemistry/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - SH2 Domain-Containing Protein Tyrosine Phosphatases
MH  - Saccharomyces cerevisiae/chemistry/genetics/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Serine Endopeptidases/genetics/metabolism
MH  - *Transcription Factors
MH  - Transcriptional Activation/genetics
MH  - ras GTPase-Activating Proteins
MH  - src Homology Domains/*genetics
EDAT- 1996/12/05 00:00
MHDA- 1996/12/05 00:01
CRDT- 1996/12/05 00:00
PHST- 1996/12/05 00:00 [pubmed]
PHST- 1996/12/05 00:01 [medline]
PHST- 1996/12/05 00:00 [entrez]
AID - S0378-1119(96)00479-9 [pii]
AID - 10.1016/s0378-1119(96)00479-9 [doi]
PST - ppublish
SO  - Gene. 1996 Dec 5;182(1-2):77-80. doi: 10.1016/s0378-1119(96)00479-9.