PMID- 8983061
OWN - NLM
STAT- MEDLINE
DCOM- 19970529
LR  - 20061115
VI  - 24
DP  - 1994
TI  - Cadherin-dependent organization and disorganization of epithelial architecture.
PG  - 28-37
AB  - Epithelial cell layers exhibit an ordered polarized architecture. However, such 
      structures are disrupted during malignant transformation, which generally 
      coincides with a loss of regulate cell growth. We are investigating how the 
      cadherin cell adhesion system controls these processes. Cadherins form a 
      molecular complex with alpha-catenin, and beta-catenin or plakoglobin at the 
      cytoplasmic side in normal cells. Lung carcinoma PC9 cells express E-cadherin. 
      Although they express other catenins, they lack alpha-catenin and cannot firmly 
      aggregate, suggesting that their E-cadherin is inactive. Transfection of the PC9 
      cells with alpha-catenin cDNA leads to activation of the E-cadherin, inducing 
      their compact aggregation. In these aggregates, an almost complete 
      epithelial-specific architecture is organized, including the formation of 
      microvilli and a junctional complex. We also studied the effect of hepatocyte 
      growth factor/scatter factor (HGF/SF) on cell-cell contacts in keratinocyte cell 
      lines, and found that this growth factor can disrupt desmosomal cell-cell 
      contacts. HGF/SF, and also epidermal growth factor, enhance tyrosine 
      phosphorylation of beta-catenin or plakoglobin in human carcinoma lines as they 
      induce scattering of these cells. These findings suggest that the cadherin 
      adhesion system is central in organizing epithelial structures and that tyrosine 
      phosphorylation of catenins may modulate this organization process.
FAU - Takeichi, M
AU  - Takeichi M
AD  - Department of Biophysics, Faculty of Science, Kyoto University, Japan.
FAU - Watabe, M
AU  - Watabe M
FAU - Shibamoto, S
AU  - Shibamoto S
FAU - Ito, F
AU  - Ito F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Princess Takamatsu Symp
JT  - Princess Takamatsu symposia
JID - 9301172
RN  - 0 (CTNNA1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Desmoplakins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (alpha Catenin)
RN  - 0 (gamma Catenin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cadherins/*physiology
MH  - Cell Adhesion
MH  - Cell Polarity
MH  - Cytoskeletal Proteins/genetics/metabolism/physiology
MH  - Desmoplakins
MH  - Desmosomes/drug effects/ultrastructure
MH  - Epidermal Growth Factor/pharmacology
MH  - *Epithelial Cells
MH  - Epithelium/metabolism
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Humans
MH  - Lung Neoplasms/pathology
MH  - Macromolecular Substances
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational
MH  - Transfection
MH  - alpha Catenin
MH  - gamma Catenin
RF  - 30
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Princess Takamatsu Symp. 1994;24:28-37.