PMID- 8983855
OWN - NLM
STAT- MEDLINE
DCOM- 19970107
LR  - 20190920
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 19
IP  - 4
DP  - 1996 Apr
TI  - cGMP is not required for capacitative Ca2+ entry in Jurkat T-lymphocytes.
PG  - 351-4
AB  - In this study, we have addressed the potential role of cGMP in regulating calcium 
      entry in Jurkat T-lymphocytes. These cells display capacitative Ca(2+)-entry in 
      response to the intracellular Ca(2+)-ATPase inhibitor, thapsigargin (TG). In the 
      presence of extracellular Ca2+, TG stimulates a sustained elevation of 
      intracellular cGMP levels. In the absence of extracellular Ca2+, TG induces no 
      apparent increase in the levels of cGMP. However, experiments using Mn2+, as a 
      surrogate for Ca2+, demonstrated that TG increased the rate of divalent cation 
      entry in the absence of extracellular Ca2+. Treatment of Jurkat cells with the 
      guanylyl cyclase inhibitor, LY83583 (20 microM), completely blocked cGMP 
      formation in response to TG. However, LY83583 treated cells still exhibited a 
      sustained, albeit partially reduced, Ca2+ response induced by TG. These data 
      demonstrate that, in Jurkat cells, the sustained formation of cGMP is dependent 
      on elevated intracellular Ca2+, and that elevated levels of cGMP are not 
      necessary for the activation of capacitative Ca2+ entry.
FAU - Bian, X
AU  - Bian X
AD  - Laboratory of Cellular and Molecular Pharmacology, National Institute of 
      Environmental Health Sciences, National Institutes of Health, Research Triangle 
      Park, North Carolina 27709, USA.
FAU - Bird, G S
AU  - Bird GS
FAU - Putney, J W Jr
AU  - Putney JW Jr
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Aminoquinolines)
RN  - 0 (Fluorescent Dyes)
RN  - 91300-60-6 (6-anilino-5,8-quinolinedione)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aminoquinolines/pharmacology
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Cyclic GMP/*pharmacology
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - T-Lymphocytes/*metabolism
EDAT- 1996/04/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S0143-4160(96)90075-5 [pii]
AID - 10.1016/s0143-4160(96)90075-5 [doi]
PST - ppublish
SO  - Cell Calcium. 1996 Apr;19(4):351-4. doi: 10.1016/s0143-4160(96)90075-5.