PMID- 8985011
OWN - NLM
STAT- MEDLINE
DCOM- 19970123
LR  - 20211203
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 275
IP  - 5297
DP  - 1997 Jan 10
TI  - Activation of SAPK/JNK by TNF receptor 1 through a noncytotoxic TRAF2-dependent 
      pathway.
PG  - 200-3
AB  - Interaction of the p55 tumor necrosis factor receptor 1 (TNF-R1)-associated 
      signal transducer TRADD with FADD signals apoptosis, whereas the TNF 
      receptor-associated factor 2 protein (TRAF2) is required for activation of the 
      nuclear transcription factor nuclear factor kappa B. TNF-induced activation of 
      the stress-activated protein kinase (SAPK) was shown to occur through a 
      noncytotoxic TRAF2-dependent pathway. TRAF2 was both sufficient and necessary for 
      activation of SAPK by TNF-R1; conversely, expression of a dominant-negative FADD 
      mutant, which blocks apoptosis, did not interfere with SAPK activation. 
      Therefore, SAPK activation occurs through a pathway that is not required for 
      TNF-R1-induced apoptosis.
FAU - Natoli, G
AU  - Natoli G
AD  - Fondazione Andrea Cesalpino and Istituto di I Clinica Medica, Policlinico Umberto 
      I, Viale del Policlinico 155, 00161 Rome, Italy.
FAU - Costanzo, A
AU  - Costanzo A
FAU - Ianni, A
AU  - Ianni A
FAU - Templeton, D J
AU  - Templeton DJ
FAU - Woodgett, J R
AU  - Woodgett JR
FAU - Balsano, C
AU  - Balsano C
FAU - Levrero, M
AU  - Levrero M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (FADD protein, human)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNF Receptor-Associated Factor 1)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 1)
RN  - EC 2.7.11.25 (MAP3K1 protein, human)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - *Adaptor Proteins, Signal Transducing
MH  - Apoptosis
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Carrier Proteins/metabolism
MH  - Cell Line
MH  - Dactinomycin/pharmacology
MH  - Enzyme Activation
MH  - Fas-Associated Death Domain Protein
MH  - Free Radical Scavengers/pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - *MAP Kinase Kinase Kinase 1
MH  - *Mitogen-Activated Protein Kinases
MH  - NF-kappa B/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Proteins/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 1
MH  - TNF Receptor-Associated Factor 2
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1997/01/10 00:00
MHDA- 1997/01/10 00:01
CRDT- 1997/01/10 00:00
PHST- 1997/01/10 00:00 [pubmed]
PHST- 1997/01/10 00:01 [medline]
PHST- 1997/01/10 00:00 [entrez]
AID - 10.1126/science.275.5297.200 [doi]
PST - ppublish
SO  - Science. 1997 Jan 10;275(5297):200-3. doi: 10.1126/science.275.5297.200.