PMID- 8986591
OWN - NLM
STAT- MEDLINE
DCOM- 19970319
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 13
IP  - 10
DP  - 1996 Nov
TI  - Efficacy of calcium ionophore A23187 oocyte activation for generating parthenotes 
      for human embryo research.
PG  - 793-6
AB  - PURPOSE: Our purpose was to examine the efficacy of Ca-A23187 to activate human 
      oocytes and produce parthenotes for research purposes. We examined the 
      feasibility of using fluorescence in situ hybridization (FISH) to study the sex 
      chromosome constitution of activated oocytes. METHODS: One hundred eight 
      nonfertilized oocytes from our IVF program were exposed to Ca-A23187. Oocyte 
      activation was determined by the presence of pronuclear (PN) development. FISH 
      was done on chromosome preparations using X and Y dual-colored probes. 
      Polyploidic and parthenogenetically activated oocytes from our IVF program served 
      as controls. RESULTS: Of the 108 oocytes, 59 (55%) had no PN, 38 (35%) one PN, 10 
      (9%) two PN, and 1 (0.9%) three PN. Fiftyseven oocytes (53%) were not recovered 
      following spreading and no chromatin was observed on 14 slides (13%) after FISH. 
      This contrasted with 50 of 227 (22%) and 3 of 227 (1.7%) loss rates, 
      respectively, for controls (P < 0.0001). Eight of 49 activated oocytes underwent 
      cleavage. FISH was performed on 37 oocytes. Of 21 zero-PN oocytes, I had no FISH 
      signals, 15 had a single X, 4 had two X's, and I had four X's. For one-PN 
      oocytes, two had no FISH signals, seven had one X, and three had two X's. For 
      two-PN oocytes, two had no FISH signals and two had two X's. FISH results were 
      consistent with a maternal origin of genetic material. CONCLUSIONS: Ca-A23187 
      resulted in a 45% activation rate, with 16% of oocytes progressing to cleavage 
      before degeneration. Oocyte activation with Ca-A23187 allowed the generation of 
      parthenotes for human embryo research. FISH was useful for evaluation of oocytes 
      and parthenotes.
FAU - Rhoton-Vlasak, A
AU  - Rhoton-Vlasak A
AD  - Department of Obstetrics and Gynecology, Mayo Clinic and Mayo Foundation, 
      Rochester, Minnesota 55905, USA.
FAU - Lu, P Y
AU  - Lu PY
FAU - Barud, K M
AU  - Barud KM
FAU - Dewald, G W
AU  - Dewald GW
FAU - Hammitt, D G
AU  - Hammitt DG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Fluorescent Dyes)
RN  - 37H9VM9WZL (Calcimycin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Blastomeres/metabolism
MH  - Calcimycin/*pharmacology
MH  - Calcium/*pharmacology
MH  - Cell Division/physiology
MH  - Cell Survival
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Oocytes/*drug effects/metabolism
MH  - Parthenogenesis/*physiology
MH  - Sex Chromosomes
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
AID - 10.1007/BF02066500 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1996 Nov;13(10):793-6. doi: 10.1007/BF02066500.