PMID- 8988402
OWN - NLM
STAT- MEDLINE
DCOM- 19970324
LR  - 20191210
IS  - 1023-3830 (Print)
IS  - 1023-3830 (Linking)
VI  - 45
IP  - 12
DP  - 1996 Dec
TI  - Effects of three different Ca(2+)-ATPase inhibitors on Ca2+ response and 
      leukotriene release in RBL-2H3 cells.
PG  - 583-9
AB  - The effects of three Ca(2+)-ATPase inhibitors, thapsigargin (TG), cyclopiazonic 
      acid (CPA), and 2,5-di(tert-butyl)-1,4-hydroquinone (DTBHQ), on the Ca2+ 
      response, degranulation, and leukotriene C4 (LTC4) release in RBL-2H3 cells were 
      investigated. All three compounds elevated the intracellular free Ca2+ 
      concentration ([Ca2+]i), and caused degranulation in the presence of 
      12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C activator. The 
      dose-dependency of each compound in the Ca2+ response was in good agreement with 
      that in degranulation. TG and CPA also caused the release of LTC4 in a 
      dose-dependent manner, and this effect was unaffected by TPA or calphostin C, a 
      selective PKC inhibitor. DTBHQ, however, did not induce LTC4 release, and rather 
      inhibited the antigen-induced release of LTC4. These results suggest [1] that 
      both degranulation and LTC4 release caused by these compounds are dependent on 
      their [Ca2+]i increasing effect, [2] that degranulation and LTC4 release are 
      mediated via independent pathways following the Ca2+ response, and [3] that DTBHQ 
      additionally prevents the synthesis of LTC4 possibly by inhibition of 
      5-lipoxygenase.
FAU - Akasaka, R
AU  - Akasaka R
AD  - Division of Biochemistry and Immunochemistry, National Institute of Health 
      Sciences, Tokyo, Japan.
FAU - Teshima, R
AU  - Teshima R
FAU - Ikebuchi, H
AU  - Ikebuchi H
FAU - Sawada, J
AU  - Sawada J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroquinones)
RN  - 0 (Indoles)
RN  - 0 (Lipoxygenase Inhibitors)
RN  - 0 (Naphthalenes)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 2CU6TT9V48 (Leukotriene C4)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - I271P23G24 (calphostin C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Animals
MH  - Basophils/drug effects/*metabolism
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Cell Degranulation/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/*toxicity
MH  - Hydroquinones/toxicity
MH  - Indoles/toxicity
MH  - Leukemia, Basophilic, Acute/pathology
MH  - Leukotriene C4/*metabolism
MH  - Lipoxygenase Inhibitors/toxicity
MH  - Mice
MH  - Naphthalenes/toxicity
MH  - Spectrometry, Fluorescence
MH  - Tetradecanoylphorbol Acetate/toxicity
MH  - Thapsigargin/toxicity
MH  - Tumor Cells, Cultured
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1007/BF02312039 [doi]
PST - ppublish
SO  - Inflamm Res. 1996 Dec;45(12):583-9. doi: 10.1007/BF02312039.