PMID- 8993975
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20041117
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 18
IP  - 1
DP  - 1997 Jan
TI  - Evaluation of MYC and chromosome 8 copy number in breast carcinoma by interphase 
      cytogenetics.
PG  - 1-7
AB  - We used fluorescence in situ hybridization (FISH) to determine MYC and chromosome 
      8 copy number on whole nuclear imprint preparations of 24 breast carcinomas, 
      seven benign breast samples, and two phyllodes tumors. None of the benign tissues 
      and neither of the phyllodes tumors demonstrated an increased copy number for MYC 
      or chromosome 8, which was defined as greater than two signals in > 10% of 
      nuclei. In contrast, 22 of 24 carcinomas demonstrated an increased MYC copy 
      number. The modal numbers of MYC copies/nucleus were 0-2 in seven cases (29%), 
      3-5 in seven cases (29%), 6-9 in five cases (21%), and > 9 in five cases (21%). 
      An increased chromosome 8 copy number was observed in 21 of 22 carcinomas with 
      MYC gain, and the modal number of signals/nucleus was either identical to (n = 
      14; 64%) or less than (n = 8; 36%) the number of MYC copies. The number of MYC 
      copies correlated with cellular DNA content, as determined by using flow 
      cytometry. In peridiploid tumors (DNA index 0.9-1.2; n = 7), the MYC copy 
      numbers/nucleus were 0-2 in five cases and 3-5 in two cases. In contrast, the 
      modal MYC copy numbers/nucleus among the 11 hyperdiploid tumors (DNA index 
      1.3-1.9) were 0-2 in one case, 3-5 in four cases, 6-9 in five cases, and > 9 in 
      one case. All three tetraploid/hypertetraploid carcinomas exhibited > 9 MYC 
      copies/nucleus. We conclude that an increased MYC copy number, as detected by 
      using interphase cytogenetics, is extremely frequent in human breast carcinomas. 
      However, in most cases, MYC gene duplication is probably secondary to polysomy of 
      chromosome 8 and/or genomic endoreduplication (i.e., DNA aneuploidy).
FAU - Visscher, D W
AU  - Visscher DW
AD  - Department of Pathology, Harper Hospital, Detroit, Michigan 48201, USA.
FAU - Wallis, T
AU  - Wallis T
FAU - Awussah, S
AU  - Awussah S
FAU - Mohamed, A
AU  - Mohamed A
FAU - Crissman, J D
AU  - Crissman JD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Aneuploidy
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosomes, Human, Pair 8/genetics
MH  - DNA, Neoplasm/*analysis
MH  - Flow Cytometry
MH  - Gene Amplification
MH  - *Genes, myc/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Phyllodes Tumor/genetics/pathology
MH  - Ploidies
EDAT- 1997/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199701)18:1<1::AID-GCC1>3.0.CO;2-7 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Jan;18(1):1-7.