PMID- 8994241
OWN - NLM
STAT- MEDLINE
DCOM- 19970328
LR  - 20081121
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 16
IP  - 12
DP  - 1996 Dec
TI  - Culture of fetal erythroid progenitor cells from maternal blood for non-invasive 
      prenatal genetic diagnosis.
PG  - 1073-82
AB  - Fetal committed erythroid progenitors CFU-E and M-BFU-E released into the 
      maternal circulation during pregnancy are ideal candidates for in vitro 
      proliferation since their lifespan is short and they can form colonies of 
      100-1000 cells in a semi-solid medium. In order to propagate these cells with a 
      high rate of purity, a strategy was devised based on their prior enrichment with 
      biotin-labelled human erythropoietin ligand and magnetic sorting before culturing 
      in a suitable medium. Eight euploid pregnancies investigated in order to address 
      this issue produced fetal clones in cultures with 18 per cent purity as assessed 
      by polymerase chain reaction (PCR) analysis for Y-specific sequences, 
      immunocytochemical staining for fetal gamma-globin, and fluorescence in situ 
      hybridization (FISH) study. The CFU-E-type colony was the most represented 
      progenitor, followed by M-BFU-E, and only occasionally was the detection of 
      CFU-GEMM recorded. The retrospective diagnosis of two cases of fetal Down's 
      syndrome by culturing fetal cells from maternal blood was accomplished for the 
      first time. FISH analysis disclosed a strong presence of fetal trisomic cells (70 
      per cent and 40 per cent in the two cases). This strong presence would suggest a 
      preferential leakage into maternal blood. The overall results of this study 
      demonstrate that fetal cells can be cultured in vitro with reliable 
      reproducibility, thus making the prospect of a non-invasive prenatal genetic 
      diagnosis realistic.
FAU - Valerio, D
AU  - Valerio D
AD  - Istituto di Ricerche Genetiche V. le della Costituzione, Centro Direzionale Is. 
      F3, Napoli, Italy.
FAU - Aiello, R
AU  - Aiello R
FAU - Altieri, V
AU  - Altieri V
FAU - Malato, A P
AU  - Malato AP
FAU - Fortunato, A
AU  - Fortunato A
FAU - Canazio, A
AU  - Canazio A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Cells, Cultured
MH  - Down Syndrome/diagnosis
MH  - Erythroid Precursor Cells/*cytology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Gestational Age
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - *Prenatal Diagnosis
EDAT- 1996/12/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0223(199612)16:12<1073::AID-PD38>3.0.CO;2-D [pii]
AID - 10.1002/(SICI)1097-0223(199612)16:12<1073::AID-PD38>3.0.CO;2-D [doi]
PST - ppublish
SO  - Prenat Diagn. 1996 Dec;16(12):1073-82. doi: 
      10.1002/(SICI)1097-0223(199612)16:12<1073::AID-PD38>3.0.CO;2-D.