PMID- 8995490
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 75
IP  - 1
DP  - 1996
TI  - Development of DNA libraries specific for Chinese hamster chromosomes 3, 4, 9, 
      10, X, and Y by DOP-PCR.
PG  - 57-62
AB  - In a previous study of ours, DNA libraries specific for Chinese hamster 
      chromosomes 1, 2, 3, 5, 6, 7, and 8 were constructed by single laser flow sorting 
      and the linker adapter PCR method. Since a single laser flow sorting of 
      chromosomes is based on chromosome size, it is difficult to separate chromosomes 
      of equal size, such as chromosome 3 and the X chromosome. This problem can be 
      circumvented by bivariate flow sorting. In the present study, we have used 
      bivariate flow sorting of Chinese hamster chromosomes and the degenerate 
      oligonucleotide-primed polymerase chain reaction (DOP-PCR) for generating DNA 
      libraries specifically for chromosomes 3, 4, 9, 10, X, and Y. Fluorescence in 
      situ hybridization (FISH) analyses indicate that the probe libraries are highly 
      specific and can be applied to various cytogenetic studies.
FAU - Xiao, Y
AU  - Xiao Y
AD  - MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      The Netherlands.
FAU - Slijepcevic, P
AU  - Slijepcevic P
FAU - Arkesteijn, G
AU  - Arkesteijn G
FAU - Darroudi, F
AU  - Darroudi F
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromosome Aberrations
MH  - *Chromosomes
MH  - Cricetinae
MH  - Cricetulus/*genetics
MH  - *DNA Probes
MH  - Flow Cytometry
MH  - *Gene Library
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mesocricetus/genetics
MH  - Polymerase Chain Reaction
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134457 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;75(1):57-62. doi: 10.1159/000134457.