PMID- 8995491
OWN - NLM
STAT- MEDLINE
DCOM- 19970130
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 75
IP  - 1
DP  - 1996
TI  - Generation of mouse chromosome painting probes by DOP-PCR amplification of 
      microdissected meiotic chromosomes.
PG  - 63-6
AB  - We describe here the development of four sets of painting probes for mouse 
      chromosomes 1 and 13, 2 and 8, 6 and 15, and X and Y by degenerate 
      oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification of 10-20 
      copies of microdissected male meiotic chromosomes. The X,Y probe was obtained 
      from the X-Y bivalent of diakinesis/metaphase I complements of mice with a normal 
      karyotype, whereas the other probes were derived from tri- or quadrivalents in 
      diakinesis/metaphase I of two reciprocal translocations, T(1;13)70H and 
      T(2;8)2Wa, and one inversion heterozygote carrying a small deletion, 
      In(6;15)Rb1Ald del6(15)1Wa. The specificity of these probes was established by 
      fluorescence in situ hybridization (FISH) to meiotic and mitotic metaphase 
      complements. The chromosomes painted by these probes could be identified by 
      single or multicolor FISH.
FAU - Xiao, Y
AU  - Xiao Y
AD  - MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      The Netherlands.
FAU - Darroudi, F
AU  - Darroudi F
FAU - Kuipers, A G
AU  - Kuipers AG
FAU - de Jong, J H
AU  - de Jong JH
FAU - de Boer, P
AU  - de Boer P
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Coloring Agents)
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - *Chromosomes
MH  - Coloring Agents
MH  - DNA Primers
MH  - *DNA Probes
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis
MH  - Mice
MH  - *Polymerase Chain Reaction
MH  - Spermatocytes
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134458 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;75(1):63-6. doi: 10.1159/000134458.