PMID- 8996548
OWN - NLM
STAT- MEDLINE
DCOM- 19970203
LR  - 20061115
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 43
IP  - 5
DP  - 1996
TI  - Comparison of fluorescent in situ hybridization (FISH) and the polymerase chain 
      reaction (PCR) for detection of residual neuroblastoma cells.
PG  - 301-3
AB  - Residual neuroblastoma (NB) cells in bone marrow or peripheral progenitor 
      hematopoietic cells harvests may be a source of relapse after autologous 
      transplantation in patients with high stage neuroblastoma. Therefore a sensitive 
      method for detecting minimal residual disease (MRD) in the harvested product is 
      important so that an appropriate purging techniques can be applied and optimized. 
      We report on the detection of NB cells by fluorescence in situ hybridization 
      (FISH) for N-myc amplification and compare the sensitivity of FISH with a 
      semiquantitative polymerase chain reaction (PCR) assay. As a model of MRD we used 
      the neuroblastoma cell line IMR-32 diluted with normal peripheral blood 
      lymphocytes. We were able to detect a single NB cell in 1000 normal mononuclear 
      cells by FISH. The PCR method, using ethidium-bromide-stained gels, required at 
      least 10% NB cells to be present for detection of an amplified band of the N-myc 
      oncogene. Thus, FISH is ten to one hundred times more sensitive in detection of 
      N-myc amplification than a differential PCR and thus it is the method of choice 
      for detection of MRD in NB patients.
FAU - Eckschlager, T
AU  - Eckschlager T
AD  - Department of Pediatric Oncology, University Hospital, Motol, Prague, Czech 
      Republic.
FAU - McClain, K
AU  - McClain K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - DNA, Neoplasm/*analysis
MH  - *Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Neoplasm, Residual
MH  - Neuroblastoma/*genetics
MH  - *Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PST - ppublish
SO  - Neoplasma. 1996;43(5):301-3.