PMID- 8996964
OWN - NLM
STAT- MEDLINE
DCOM- 19970226
LR  - 20041117
IS  - 0916-8478 (Print)
IS  - 0916-8478 (Linking)
VI  - 41
IP  - 3
DP  - 1996 Sep
TI  - Estimation of the physical distance between major genomic markers in the Werner 
      syndrome locus (8p11.2-12) by dual-color fish analysis.
PG  - 291-7
AB  - The gene responsible for Werner syndrome (WS) is considered to be located between 
      D8S131 and D8S87 in the 8p11.2-12 region that includes the closest marker D8S339 
      (Goto et al. (1992) Nature 355: 735-738). In this experiment, the order of major 
      markers in this region was determined and the physical distances between them 
      were estimated by dual-color fluorescence in situ hybridization (FISH) using P1, 
      PAC and cosmid clone DNAs as probes. The fine overall order of 
      telomere-D8S131-D8S339-GSR-PP2A beta-D8S283-D8S87-centromere was determined for 
      the first time. The distance from D8S131 to D8S87 was estimated to be 1,634 kb. 
      To our surprise, the distance between D8S131 and D8S87 is much shorter than 
      previously estimated by recombination analysis, 8.3 cM equivalent to 8.3 Mb in 
      physical distance. These information provide the basis for the positional cloning 
      of WS gene and the identification of its mutation.
FAU - Tokutake, Y
AU  - Tokutake Y
AD  - AGENE Research Institute, Kamakura, Japan.
FAU - Satoh, M
AU  - Satoh M
FAU - Suzuki, N
AU  - Suzuki N
FAU - Sugimoto, M
AU  - Sugimoto M
FAU - Sugawara, M
AU  - Sugawara M
FAU - Goto, M
AU  - Goto M
FAU - Furuichi, Y
AU  - Furuichi Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Hum Genet
JT  - The Japanese journal of human genetics
JID - 9213239
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/chemistry
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Conformation
MH  - Werner Syndrome/*genetics
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 10.1007/BF01913171 [doi]
PST - ppublish
SO  - Jpn J Hum Genet. 1996 Sep;41(3):291-7. doi: 10.1007/BF01913171.