PMID- 8997490
OWN - NLM
STAT- MEDLINE
DCOM- 19970410
LR  - 20081121
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 377
IP  - 12
DP  - 1996 Dec
TI  - DNA-binding specificity of PAR and C/EBP leucine zipper proteins: a single amino 
      acid substitution in the C/EBP DNA-binding domain confers PAR-like specificity to 
      C/EBP.
PG  - 797-809
AB  - PAR and C/EBP family proteins are liver-enriched basic leucine zipper (bZip) 
      transcription factors that bind similar sites on the promoters of albumin and 
      cholesterol 7 alpha hydroxylase genes. However, C/EBP proteins have a more 
      relaxed binding specificity than PAR proteins, in that they recognize many sites 
      within promoter or randomly selected rat genomic DNA sequences that are ignored 
      by PAR proteins. Thus, DNAse I protection experiments suggest that C/EBP 
      recognizes a binding site with an affinity similar to the one of the cholesterol 
      7 alpha hydroxylase gene promoter every 200 to 300 bp. The frequency of PAR 
      protein binding sites with comparable affinities is about 20-fold lower in the 
      rat genome. By using a PCR-based amplification assay we selected high affinity 
      DNA-binding sites for C/EBP beta and the PAR protein DBP from a pool of 
      oligonucleotides. Both proteins indeed recognize similar sequences with the 
      optimal core binding sequences 5'RTTAY.GTAAY3'. However, as expected, DBP, is 
      considerably less tolerant to deviations from the consensus site. Here we have 
      characterized a single amino acid substitution mutant of C/EBP beta that 
      increases its target site specificity. This protein, C/EBP beta V > A, contains a 
      valine to alanine substitution at position 13 of the basic domain (residue 216 of 
      C/EBP beta). C/EBP beta V > A selectively binds only the subset of C/EBP sites 
      that are also DBP sites, both as oligonucleotides and within the natural contexts 
      of the albumin and cholesterol hydroxylase promoters.
FAU - Falvey, E
AU  - Falvey E
AD  - Department of Molecular Biology, University of Geneva, Switzerland.
FAU - Marcacci, L
AU  - Marcacci L
FAU - Schibler, U
AU  - Schibler U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DBP protein, rat)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CCAAT-Enhancer-Binding Proteins
MH  - DNA Footprinting
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Leucine Zippers
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Sequence Alignment
MH  - Structure-Activity Relationship
MH  - Transcription Factors/chemistry/*metabolism
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Biol Chem. 1996 Dec;377(12):797-809.