PMID- 9003525
OWN - NLM
STAT- MEDLINE
DCOM- 19970220
LR  - 20091119
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 372
IP  - 1
DP  - 1996 Nov 11
TI  - Frequencies of X-ray induced pericentric inversions and centric rings in human 
      blood lymphocytes detected by FISH using chromosome arm specific DNA libraries.
PG  - 1-7
AB  - Frequencies of intra-chromosomal exchanges (pericentric inversions and centric 
      rings) and inter-chromosomal exchanges (dicentrics and translocations) in 
      X-irradiated (2.5 Gy) human lymphocytes have been estimated. To detect these 
      events we employed FISH (fluorescence in situ hybridization) technique and arm 
      specific painting probes for chromosomes #1 and #3. The ratio between centric 
      rings and pericentric inversions was found to be about 1. For intra-changes to 
      inter-changes, the ratio (F) was between 6 and 9. Based on the total number of 
      colour junctions involving chromosomes #1 and #3 it was found that exchanges 
      between the arms of the same chromosome occur about 8.7 times more than 
      inter-chromosomal exchanges calculated on the basis of the DNA content of the 
      chromosomes and random induction of aberrations in the total genome. Chromosomal 
      organization in interphase nucleus appears to promote the formation of more 
      intra-changes than inter-changes following X-irradiation, most probably due to 
      close proximity of the two arms of a chromosome.
FAU - Natarajan, A T
AU  - Natarajan AT
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      Netherlands. Natarajan@rullf2.medfac.leidenuniv.nl
FAU - Boei, J J
AU  - Boei JJ
FAU - Vermeulen, S
AU  - Vermeulen S
FAU - Balajee, A S
AU  - Balajee AS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Inversion
MH  - Chromosomes, Human/*radiation effects
MH  - Chromosomes, Human, Pair 1/radiation effects
MH  - Chromosomes, Human, Pair 3/radiation effects
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/*radiation effects
MH  - Male
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic
MH  - X-Rays
EDAT- 1996/11/11 00:00
MHDA- 1996/11/11 00:01
CRDT- 1996/11/11 00:00
PHST- 1996/11/11 00:00 [pubmed]
PHST- 1996/11/11 00:01 [medline]
PHST- 1996/11/11 00:00 [entrez]
AID - S0027-5107(96)00209-6 [pii]
AID - 10.1016/S0027-5107(96)00209-6 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Nov 11;372(1):1-7. doi: 10.1016/S0027-5107(96)00209-6.