PMID- 9003602
OWN - NLM
STAT- MEDLINE
DCOM- 19970325
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 35
IP  - 2
DP  - 1997 Feb
TI  - Clonal dissemination and colony morphotype variation of vancomycin-resistant 
      Enterococcus faecium isolates in metropolitan Detroit, Michigan.
PG  - 388-92
AB  - Thirty-two isolates of vancomycin-resistant Enterococcus faecium (VRE) recovered 
      from 25 patients hospitalized at six hospitals in the metropolitan Detroit, 
      Mich., area over a 32-month period were examined for relatedness by 
      repetitive-sequence PCR (rep-PCR). All isolates were shown to carry the vanA gene 
      by PCR. The rep-PCR patterns generated from each isolate showed that the first 
      three VRE isolates obtained from hospital A between June 1992 and February 1994 
      were distinct strains. Thereafter, all VRE isolates originating from hospital A 
      and those collected from five other area hospitals had identical rep-PCR 
      patterns. On detailed examination, subcultures of 25 of the 32 VRE isolates 
      produced two distinct colony types characterized phenotypically by a rough and a 
      smooth appearance, respectively. Both colony types retained the vanA locus and 
      the rep-PCR pattern of the primary isolate. These data suggest that a single 
      strain of VRE with the capacity to produce two colonial variants has been 
      disseminated to several Detroit-area hospitals. The clinical significance of the 
      colonial morphotypes is unclear.
FAU - Dunne, W M Jr
AU  - Dunne WM Jr
AD  - Henry Ford Health System, Detroit, Michigan 48202, USA.
FAU - Wang, W
AU  - Wang W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (VanA ligase, Bacteria)
RN  - 6Q205EH1VU (Vancomycin)
RN  - EC 6.- (Ligases)
RN  - EC 6.1.- (Carbon-Oxygen Ligases)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Proteins/genetics
MH  - Bacterial Typing Techniques
MH  - *Carbon-Oxygen Ligases
MH  - Drug Resistance, Microbial/genetics
MH  - Enterococcus faecium/classification/*cytology/*drug effects/genetics
MH  - Gram-Positive Bacterial Infections/*microbiology
MH  - Humans
MH  - Ligases/genetics
MH  - Michigan
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Vancomycin/*pharmacology
PMC - PMC229586
EDAT- 1997/02/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1997/02/01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PHST- 1997/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.35.2.388-392.1997 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1997 Feb;35(2):388-92. doi: 10.1128/jcm.35.2.388-392.1997.