PMID- 9008725
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 371
IP  - 3-4
DP  - 1996 Dec 20
TI  - Quantification of epithelial cell micronuclei by fluorescence in situ 
      hybridization (FISH) in mortuary science students exposed to formaldehyde.
PG  - 237-48
AB  - A micronucleus assay employing fluorescence in situ hybridization (FISH) with a 
      centromeric probe was used on specimens of exfoliated buccal and nasal cells 
      collected from mortuary science students exposed to embalming fluid containing 
      formaldehyde. FISH labeling allowed micronuclei (MN) containing a whole 
      chromosome (centromere-positive, MN+) to be differentiated from those containing 
      only chromosomal fragments (centromere-negative, MN-). Each student was sampled 
      before and after the 90 day embalming class. We determined if an increase in MN 
      frequency could be attributed to formaldehyde exposure and was specific to either 
      MN+ or MN-. In buccal cells, total MN frequency was significantly increased from 
      0.6/1000 to 2/1000 (p = 0.007) following the course, whereas in nasal cells it 
      was not (2 and 2.5/1000, respectively, p = 0.2). Cells with multiple MN were 
      present only in samples taken after exposure to embalming fluid. Although the 
      baseline frequency was higher for MN+ in both buccal (0.4/1000 for MN+ and 
      0.1/1000 for MN-) and nasal cells (1.2/1000 for MN+ and 0.5/1000 for MN-), the 
      increase in MN frequency was greater for MN-, (9-fold, p = 0.005 for buccal 
      cells; 2-fold, p = 0.03 for nasal cells) than for MN+ (> 2-fold, p = 0.08 for 
      buccal cells; no change, p = 0.31 for nasal cells) in both tissues. Thus, the 
      primary mechanism of micronucleus formation appeared to be chromosome breakage. 
      This finding is consistent with known clastogenic properties of formaldehyde, the 
      component of embalming fluid most likely responsible for micronucleus induction.
FAU - Titenko-Holland, N
AU  - Titenko-Holland N
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California at Berkeley 94720, USA. ninah@uclink4.berkeley.edu
FAU - Levine, A J
AU  - Levine AJ
FAU - Smith, M T
AU  - Smith MT
FAU - Quintana, P J
AU  - Quintana PJ
FAU - Boeniger, M
AU  - Boeniger M
FAU - Hayes, R
AU  - Hayes R
FAU - Suruda, A
AU  - Suruda A
FAU - Schulte, P
AU  - Schulte P
LA  - eng
GR  - P30 ES01896/ES/NIEHS NIH HHS/United States
GR  - P42-ES04705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Adult
MH  - Female
MH  - Formaldehyde/*toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Micronucleus Tests
MH  - Mortuary Practice/*education
MH  - Mouth Mucosa/drug effects
MH  - Nasal Mucosa/drug effects
MH  - *Occupational Exposure
MH  - Quality Control
EDAT- 1996/12/20 00:00
MHDA- 1996/12/20 00:01
CRDT- 1996/12/20 00:00
PHST- 1996/12/20 00:00 [pubmed]
PHST- 1996/12/20 00:01 [medline]
PHST- 1996/12/20 00:00 [entrez]
AID - S0165-1218(96)90112-3 [pii]
AID - 10.1016/s0165-1218(96)90112-3 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Dec 20;371(3-4):237-48. doi: 10.1016/s0165-1218(96)90112-3.