PMID- 9009081
OWN - NLM
STAT- MEDLINE
DCOM- 19970224
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 2
DP  - 1997 Feb
TI  - Clonality analysis of hematopoietic cell lineages in acute myeloid leukemia and 
      translocation (8;21): only myeloid cells are part of the malignant clone.
PG  - 202-5
AB  - Bone marrow from six patients with acute myeloid leukemia (AML) and t(8;21) 
      (q22;q22) or a variant t(8;13;21) was studied by simultaneous analysis of cell 
      morphology and karyotype. Combination of May-Grunwald-Giemsa (MGG) and 
      fluorescence in situ hybridization (FISH) using probes specific for the 
      breakpoint regions of chromosome 8 and 21 allowed us to establish the extent of 
      cell-lineage involvement of the translocation. The translocation was found in all 
      myeloid blasts and in high percentages of the more mature neutrophilic cells. In 
      one patient we could demonstrate the translocation in the eosinophils as well. 
      Erythroblasts and lymphocytes did not show the t(8;21) abnormality. These results 
      indicate that the t(8;21) in AML is restricted to the myeloid (granulocytic) 
      lineage.
FAU - van Lom, K
AU  - van Lom K
AD  - Department of Hematology, University Hospital Rotterdam and Erasmus University 
      Rotterdam, The Netherlands.
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Vandekerckhove, F
AU  - Vandekerckhove F
FAU - de Greef, G E
AU  - de Greef GE
FAU - Sacchi, N
AU  - Sacchi N
FAU - Lowenberg, B
AU  - Lowenberg B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
EIN - Leukemia 1997 Jun;11(6):896
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow/pathology
MH  - Cell Differentiation/drug effects
MH  - Chromosomes, Human, Pair 21/*ultrastructure
MH  - Chromosomes, Human, Pair 8/*ultrastructure
MH  - Clone Cells/*pathology
MH  - Female
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Stem Cells/*pathology
MH  - *Translocation, Genetic
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1038/sj.leu.2400544 [doi]
PST - ppublish
SO  - Leukemia. 1997 Feb;11(2):202-5. doi: 10.1038/sj.leu.2400544.