PMID- 9010102
OWN - NLM
STAT- MEDLINE
DCOM- 19970221
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 79
IP  - 2
DP  - 1997 Jan 15
TI  - Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in 
      situ hybridization and DNA flow cytometry using paraffin-embedded tissue.
PG  - 284-93
AB  - BACKGROUND: Myxoid liposarcoma is one of the major myxoid malignancies of soft 
      tissue and its histologic differentiation from other myxoid tumors is sometimes 
      difficult. Recent cytogenetic analysis revealed that a characteristic reciprocal 
      chromosomal translocation of t(12;16)(q13;p11) occurs in myxoid liposarcomas. In 
      this study, retrospective cytogenetic analysis by means of fluorescence in situ 
      hybridization (FISH) and DNA flow cytometry were performed on free nuclei 
      isolated from paraffin embedded myxoid liposarcoma cells, and they were compared 
      with myxoid malignant fibrous histiocytomas (MFHs), low grade fibromyxoid 
      sarcomas, and intramuscular myxomas. METHODS: Nine myxoid liposarcomas, five 
      myxoid MFHs, two low grade fibromyxoid sarcomas, and four intramuscular myxomas 
      were examined. Chromosomal aberrations in chromosomes 12 and 16 were investigated 
      by FISH, using both centromeric DNA probes and whole chromosome painting probes. 
      Cellular DNA contents were determined by flow cytometry. RESULTS: All nine myxoid 
      liposarcomas were shown to be diploid by DNA flow cytometry and exhibited the 
      translocation t(12; 16), as shown by FISH. Four of the five myxoid MFHs exhibited 
      aneuploidy, as shown by flow cytometry, and remarkable numeric aberrations of 
      chromosomes 12 and 16. No abnormal DNA content or chromosomal aberrations were 
      identified in the two low grade fibromyxoid sarcomas or the four intramuscular 
      myxomas. CONCLUSIONS: FISH analysis performed on paraffin embedded materials can 
      be used to differentiate myxoid liposarcomas from other myxoid soft tissue 
      tumors.
FAU - Aoki, T
AU  - Aoki T
AD  - Department of Pathology and Oncology, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Hisaoka, M
AU  - Hisaoka M
FAU - Kouho, H
AU  - Kouho H
FAU - Hashimoto, H
AU  - Hashimoto H
FAU - Nakata, H
AU  - Nakata H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Flow Cytometry
MH  - Histiocytoma, Benign Fibrous/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Liposarcoma, Myxoid/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Muscle Neoplasms/genetics
MH  - Soft Tissue Neoplasms/*genetics/pathology
EDAT- 1997/01/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/01/15 00:00
PHST- 1997/01/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/01/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-0142(19970115)79:2<284::AID-CNCR12>3.0.CO;2-X [pii]
AID - 10.1002/(sici)1097-0142(19970115)79:2<284::aid-cncr12>3.0.co;2-x [doi]
PST - ppublish
SO  - Cancer. 1997 Jan 15;79(2):284-93. doi: 
      10.1002/(sici)1097-0142(19970115)79:2<284::aid-cncr12>3.0.co;2-x.