PMID- 9010447
OWN - NLM
STAT- MEDLINE
DCOM- 19970224
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 76
IP  - 1
DP  - 1997 Jan
TI  - Characterization of a newly established human bone marrow endothelial cell line: 
      distinct adhesive properties for hematopoietic progenitors compared with human 
      umbilical vein endothelial cells.
PG  - 25-36
AB  - Human bone marrow endothelial cells (HBMEC) are intimately involved in the homing 
      of hematopoietic progenitor cells (HPC) to the bone marrow and in the regulation 
      of proliferation and differentiation of these cells. Because availability of 
      primary HBMEC and their capacity to be cultured in vitro are limited, we used 
      isolated HBMEC to establish a cloned cell line by microinjection of a recombinant 
      plasmid expressing simian virus 40 early genes under the control of a deletion 
      mutant of the human vimentin promoter. Serum requirements for growth of a 
      transformed HBMEC line (TrHBMEC) were markedly decreased compared with those of 
      primary cells, and added growth factors were not required for proliferation. 
      Cells took up acetylated low-density lipoprotein normally, bound to Ulex 
      europaeus lectin, and stained positively for von Willebrand factor, P-selectin, 
      CD31, CD34, CD44, very late antigen-5, and intercellular adhesion molecule-2 
      (ICAM-2). After treatment with TNF-alpha or lipopolysaccharide, TrHBMEC increased 
      surface expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1), and 
      ICAM-1 in a manner similar to primary HBMEC. In contrast, IL-1 beta elicited much 
      less up-regulation of these adhesion molecules than in primary cells. In previous 
      work, we reported that, in a flow adhesion model, rolling of peripheral blood 
      CD34+ cells on primary HBMEC was E-selectin-dependent, whereas VCAM-1 and ICAM-1 
      contributed to firm adhesion. In the present study, we show that HPC adhere in a 
      similar way to TrHBMEC. A less-pronounced role for VCAM-1 and ICAM-1 was found in 
      the adhesion of HPC to human umbilical vein endothelial cells. Furthermore, 
      significantly more CD34+ cells adhered to TNF-alpha-stimulated HBMEC and TrHBMEC 
      than to similarly stimulated human umbilical vein endothelial cells. These data 
      emphasize the importance of using microvessel HBMEC for studying the homing of 
      HPC to the bone marrow, and indicate the usefulness of the above-described bone 
      marrow endothelial cell line.
FAU - Schweitzer, K M
AU  - Schweitzer KM
AD  - Department of Hematology, Free University Hospital Amsterdam, The Netherlands.
FAU - Vicart, P
AU  - Vicart P
FAU - Delouis, C
AU  - Delouis C
FAU - Paulin, D
AU  - Paulin D
FAU - Drager, A M
AU  - Drager AM
FAU - Langenhuijsen, M M
AU  - Langenhuijsen MM
FAU - Weksler, B B
AU  - Weksler BB
LA  - eng
GR  - HL-18828/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vimentin)
SB  - IM
MH  - Antigens, CD/analysis/*biosynthesis
MH  - Antigens, CD34/analysis
MH  - Antigens, Viral, Tumor/biosynthesis
MH  - Bone Marrow/physiology
MH  - *Bone Marrow Cells
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/analysis/*biosynthesis
MH  - Cell Line
MH  - Clone Cells
MH  - Culture Techniques/methods
MH  - Endothelium/*cytology/physiology
MH  - Endothelium, Vascular/cytology/*physiology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/cytology/*physiology
MH  - Humans
MH  - Immunoglobulin G
MH  - Regulatory Sequences, Nucleic Acid
MH  - Simian virus 40/genetics
MH  - Transfection
MH  - Umbilical Veins
MH  - Vimentin/biosynthesis
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1997 Jan;76(1):25-36.